In vitro biocompatibility screening of a colloidal gum Arabic-polyaniline conducting nanocomposite

Autor(es)

Goncalves, Jenifer Pendiuk ^[1] ; de Oliveira, Carolina Camargo ^[1] ; Trindade, Edvaldo da Silva ^[1] ; Riegel-Vidotti, Izabel Cristina ^[2] ; Vidotti, Marcio ^[2] ; Simas, Fernanda Fogagnoli ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Parana UFPR, Cell Biol Dept, Sect Biol Sci, Lab Inflammatory & Neoplast Cells, Lab Sulfated Po, Av Cel Francisco H dos Santos S-N, BR-81530980 Curitiba, Parana - Brazil

^[2] UFPR, Dept Chem, Macromol & Interfaces Res Grp, Av Cel Francisco H dos Santos S-N, BR-81530980 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 173, p. 109-117, MAR 15 2021.
• Citações Web of Science: 0

Resumo

Although polyaniline (PANI) is a widely investigated conductive polymer for biological applications, studies addressing the biocompatibility of colloidal PANI dispersions are scarcely found in the literature of the area. Therefore, PANI nanoparticles stabilized by the natural polysaccharide gum Arabic (GA) were screened for their biocompatibility. The GA successfully stabilized the colloidal PANI-GA dispersions when exposed to a protein-rich medium, showing compatibility with the biological environment. The results obtained from a series of in vitro assays showed that, after up to 48 h of exposure to a range of PANI-GA concentrations (1-50 mu g/mL), both mouse BALB/3T3 fibroblasts and RAW 264.7 macrophages showed no evidence of change in cellular proliferation, viability and metabolic activity. An increase in macrophage granularity poses as evidence of phagocytic uptake of PANI-GA, without resulting activation of this cell type. Additionally, the PANI-GA nanoparticles modulated the cell morphology changes induced on fibroblasts by GA in a concentration-dependent manner. Thus, this unprecedented biocompatibility study of PANI nanoparticles stabilized by a plant gum exudate polysaccharide showed promising results. This simple biomaterial might be further developed into colloidal formulations for biological and biomedical applications, taking advantage of its versatility, biocompatibility, and conductive properties. (C) 2021 Published by Elsevier B.V. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático