Morphological descriptions of the nymph and adults of Ornithodoros clarki, the larva and nymph of Ornithodoros rondoniensis, with notes on their phylogenetic relationships

Autor(es)

Labruna, Marcelo B. ^[1] ; Nava, Santiago ^[2] ; Rebollo-Hernandez, Andrea ^[3] ; Guzman-Cornejo, Carmen ^[3] ; Hernandez, Ligia V. ^[4] ; Dominguez, Lillian ^{[5, 6]} ; Munoz-Leal, Sebastian ^[7] ; Venzal, Jose M.

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Av Prof Orlando Marques Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] INTA Rafaela, EEA, CONICET, IDICAL, INTA, Rafaela, Santa Fe - Argentina

^[3] Univ Nacl Autonoma Mexico, Dept Biol, Fac Ciencias, Lab Acarol, Mexico City, DF - Mexico

^[4] Univ Nacl Autonoma Nicaragua, Escuela Ciencias Agr & Vet, Campus Agr Leon, AP 68, Leon - Nicaragua

^[5] Inst Conmemorat Gorgas Estudios Salud, Dept Invest Entomol Med, Panama City - Panama

^[6] AIP, Estn Cient Coiba, Coiba - Panama

^[7] Univ Concepcion, Fac Ciencias Vet, Dept Patol & Med Prevent, Chillan, Nuble - Chile

• Tipo de documento: Artigo Científico
• Fonte: Systematic Parasitology; v. 98, n. 3 MAR 2021.
• Citações Web of Science: 0

Resumo

Based on tick specimens collected recently in Mexico, Nicaragua, Panama and Brazil, we provide morphological descriptions of the nymph and adults of Ornithodoros clarki Jones \& Clifford, 1972 from the first three countries, and the larva and nymph of Ornithodoros rondoniensis (Labruna, Terassini, Camargo, Brandao, Ribeiro \& Estrada-Pena, 2008) from Brazil. Also, an analysis of mitochondrial 16S rDNA sequences was performed to analyze the phylogenetic relationships of these tick species. Adults and nymphs of O. clarki and O. rondoniensis are unique among the Argasidae family by presenting exceptionally large spiracular plates with small goblets, and an integument with smooth polygonal mammillae. However, these two species are morphologically distinct based on specific patterns of coxal folds, idiosomal mammillae and pilosity, and female genital flap. In contrast, the larvae of O. clarki and O. rondoniensis are morphologically identical, except for a general larger size of the former species; this slight difference is corroborated by Principal Component Analysis (PCA) by using 40 morphometric variables. Phylogenetic analyses including 16S rDNA partial sequences of different Ornithodoros taxa from Central and South America indicate that O. rondoniensis from Brazil diverges from O. clarki from Mexico, Nicaragua and Panama. However, phylogenetic distance separating both alleged species is similar or slightly lower than the distances depicted for conspecific populations of a few other Ornithodoros species. Nonetheless, our primary criterion to maintain O. rondoniensis as a valid species is because its adult and nymphal stages do present distinct morphological traits that easily distinguish these postlarval stages from O. clarki. (AU)

• Processo FAPESP: 18/02521-1 - Pesquisa de espiroquetas do grupo da febre recorrente (Spirochaetaceae: Borrelia) em carrapatos do gênero Ornithodoros (Acari: Argasidae) parasitas de humanos no Brasil
• Beneficiário: Sebastián Alejandro Munoz Leal
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado