Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment

Autor(es)

Aissaoui, Salima ^[1] ; Fagnani, Enelton ^{[2, 3]} ; Perez, Sandra ^[3] ; Ouled-Haddar, Houria ^[1] ; Sifour, Mohamed ^[1]

Afiliação do(s) autor(es)

^[1] Univ Mohammed Seddik Benyahia Jijel, Fac Nat & Life Sci, Lab Mol Toxicol, Jijel - Algeria

^[2] CSIC, IDAEA, Dept Environm Chem, ENFOCHEM, Jordi Girona 18-26, Barcelona 08034 - Spain

^[3] Univ Campinas UNICAMP, Sch Technol, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 52, n. 2 MAR 2021.
• Citações Web of Science: 0

Resumo

Diclofenac (DCF) belongs to the class of nonsteroidal anti-inflammatory drugs, which is one of the most consumed by population and detected in raw sewage. Several studies have reported variable removal rates by biodegradation of diclofenac in wastewater treatment plants (WWTPs). This study deals with the evaluation of the biodegradation of DCF by a bacterial consortium (obtained from pure cultures of Enterobacter hormaechei D15 and Enterobacter cloacea D16), which were isolated from household compost and Algerian WWTP, respectively, as sole carbon source and by co-metabolism, using glucose as carbon source. A 98% removal rate of DCF was observed when it is used as the sole carbon source, whilst only 44% of DCF was removed in co-metabolic conditions. Two metabolites were identified using ultra-high-performance liquid chromatography coupled to electrospray injection tandem mass spectrometry analysis (UHPLC-ESI-MS/MS); one of them was identified as 4 `-hydroxy-DCF, and the second metabolite was suspected to be a nitro derivative of DCF, according to comparison with the literature. Biodegradation of DCF by this bacterial consortium generates relatively safe final by-products. (AU)

• Processo FAPESP: 18/09875-3 - Detecção de fármacos halogenados e seus produtos de transformação em ecossistemas aquáticos fluviais por cromatografia líquida acoplada a espectrometria de massas de alta resolução (LC-HRMS), usando-se a abordagem de investigação de compostos suspeitos
• Beneficiário: Enelton Fagnani
• Modalidade de apoio: Bolsas no Exterior - Pesquisa