Suturable elastomeric tubular grafts with patterned porosity for rapid vascularization of 3D constructs

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Bellani, Caroline Faria ^{[1, 2, 3, 4, 5]} ; Yue, Kan ^{[4, 5, 6]} ; Flaig, Florence ^[2] ; Hebraud, Anne ^[2] ; Ray, Pengfei ^{[4, 5]} ; Annabi, Nasim ^{[7, 4, 5, 8]} ; Selistre de Araujo, Heloisa Sobreiro ^[3] ; Branciforti, Marcia Cristina ^{[1, 9]} ; Minarelli Gaspar, Ana Maria ^[10] ; Shin, Su Ryon ^{[4, 5]} ; Khademhosseini, Ali ^{[7, 4, 5, 8, 11]} ; Schlatter, Guy ^[2]

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^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Bioengn Dept, Sao Carlos, SP - Brazil

^[2] Univ Strasbourg, UMR 7515 CNRS, ECPM, Inst Chim & Procedes Energie Environm & Sante ICP, Strasbourg - France

^[3] Univ Fed Sao Carlos, Physiol Sci Dept, Lab Biochem & Mol Biol, Sao Carlos - Brazil

^[4] MIT, Harvard Massachusetts Inst Technol, Div Hlth Sci & Technol, Cambridge, MA 02139 - USA

^[5] Harvard Med Sch, Dept Med, Brigham & Womens Hosp, Boston, MA 02139 - USA

^[6] South China Univ Technol, South China Adv Inst Soft Matter Sci & Technol, Guangzhou - Peoples R China

^[7] Univ Calif Los Angeles, Ctr Minimally Invas Therapeut C MIT, Los Angeles, CA 90095 - USA

^[8] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 - USA

^[9] Univ Sao Paulo, Mat Engn Dept, Sao Carlos Sch Engn, Sao Carlos, SP - Brazil

^[10] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Morphol, Araraquara, SP - Brazil

^[11] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 - USA

• Tipo de documento: Artigo Científico
• Fonte: BIOFABRICATION; v. 13, n. 3 JUL 2021.
• Citações Web of Science: 0

Resumo

Vascularization is considered to be one of the key challenges in engineering functional 3D tissues. Engineering suturable vascular grafts containing pores with diameter of several tens of microns in tissue engineered constructs may provide an instantaneous blood perfusion through the grafts improving cell infiltration and thus, allowing rapid vascularization and vascular branching. The aim of this work was to develop suturable tubular scaffolds to be integrated in biofabricated constructs, enabling the direct connection of the biofabricated construct with the host blood stream, providing an immediate blood flow inside the construct. Here, tubular grafts with customizable shapes (tubes, Y-shape capillaries) and controlled diameter ranging from several hundreds of microns to few mm are fabricated based on poly(glycerol sebacate) (PGS)/poly(vinyl alcohol) (PVA) electrospun scaffolds. Furthermore, a network of pore channels of diameter in the order of 100 mu m was machined by laser femtosecond ablation in the tube wall. Both non-machined and laser machined tubular scaffolds elongated more than 100% of their original size have shown suture retention, being 5.85 and 3.96 N mm(-2) respectively. To demonstrate the potential of application, the laser machined porous grafts were embedded in gelatin methacryloyl (GelMA) hydrogels, resulting in elastomeric porous tubular graft/GelMA 3D constructs. These constructs were then co-seeded with osteoblast-like cells (MG-63) at the external side of the graft and human umbilical vein endothelial cells inside, forming a bone osteon model. The laser machined pore network allowed an immediate endothelial cell flow towards the osteoblasts enabling the osteoblasts and endothelial cells to interact and form 3D structures. This rapid vascularization approach could be applied, not only for bone tissue regeneration, but also for a variety of tissues and organs. (AU)

• Processo FAPESP: 16/04418-8 - Enxertos vasculares tubulares obtidos por eletrofiação para vascularização rápida de construtos para engenharia tecidual óssea
• Beneficiário: Caroline Faria Bellani
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 14/17939-0 - Desenvolvimento de scaffolds de poli(caprolactona) incorporados com biosilicato e nanocristais de celulose para Engenharia de Tecidos e regeneração óssea
• Beneficiário: Caroline Faria Bellani
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto