Proteasome activity is altered in skeletal muscle tissue of tumour-bearing rats a leucine-rich diet

Autor(es)

Mello, M. A. R. ; Gomes-Marcondes, M. C. C. ; Ventrucci, G. ^[3]

• Tipo de documento: Artigo Científico
• Fonte: Endocrine-Related Cancer; v. 11, n. 4, p. 887-895, Nov. 2004.
• Área do conhecimento: Ciências da Saúde - Nutrição
• Assunto(s): Suplementação alimentar Neoplasias

Resumo

Leucine can modulate skeletal muscle metabolism by enhancing protein synthesis and decreasing proteolysis. In this study, we investigated the effects of leucine on the ubiquitin-proteasome system in skeletal muscle of pregnant tumour-bearing rats fed a leucine-rich diet. Pregnant Wistar rats were distributed into three groups that were fed a semi-purified control diet (C, control; W, Walker tumour-bearing; P, pair-fed) and three other groups of pregnant rats fed a semi-purified leucine-rich diet (L, leucine; WL, Walker tumour-bearing; PL, pair-fed). The tumour-bearing rats were injected subcutaneously with a suspension of Walker 256 tumour cells. Protein synthesis and degradation were measured in gastrocnemius muscle; the total protein content and tissue chymotrypsin-like and alkaline phosphatase enzyme activities were also determined. Muscle protein extracts were run on SDS-PAGE to assess the expression of the myosin heavy chain (MHC), 20S proteasome subunit, 19S MSSI ATPase regulator subunit and 11S subunit. Although tumour growth decreased the incorporation of [3H]-Phe, the concomitant feeding of a leucine-rich diet increased the rate of protein synthesis. Muscle proteolysis in both tumour-bearing groups was increased more than in the respective control groups. Conversely, the leucine-rich diet caused less protein breakdown in the WL group than in the W group. Only the W group showed a significant reduction (71%) in the myosin content. In WL rats, the 20S proteasome content (32 kDa band) was reduced, while the expression of the 19S subunit was 3-fold less than in the W group and the 11S proteasome subunit reduced, to around 32% less than in the W group. These findings clearly indicate that leucine can stimulate protein synthesis and inhibit protein breakdown in pregnant rats, probably by modulating the activation of the ubiquitin-proteasome system during tumour growth. (AU)

• Processo FAPESP: 02/04464-7 - Estudo dos processos metabólicos e moleculares da caquexia em ratas grávidas ou não, implantadas com tumor de Walker 256
• Beneficiário: Gislaine Ventrucci
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 96/09463-6 - Correlação entre câncer, gravidez e nutrição: estudo experimental sobre as alterações no metabolismo protéico em ratos jovens
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/02135-3 - Efeito da associação entre nutrição, câncer e gravidez: estudo dos processos metabólicos e moleculares da caquexia in vivo in vitro
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular