Follow-up of dairy cattle naturally infected by Trypanosoma vivax after treatment with isometamidium chloride

Autor(es)

de Almeida Castilho Neto, Kayo Jose Garcia ^[1] ; da Cruz Favaro Garcia, Ana Beatriz ^[1] ; Fidelis Junior, Otavio Luiz ^[2] ; Nagata, Walter Beterquini ^[3] ; Andre, Marcos Rogerio ^[1] ; Geraldes Teixeira, Marta Maria ^[4] ; Machado, Rosangela Zacarias ^[1] ; Cadioli, Fabiano Antonio ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet FCAV, UNESP, Jaboticabal, SP - Brazil

^[2] Univ Vila Velha UVV, Dept Med Vet, Vila Velha, ES - Brazil

^[3] Univ Estadual Paulista, UNESP, Escola Med Vet Med, Aracatuba, SP - Brazil

^[4] Univ Sao Paulo, Inst Ciencias Biomed, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 30, n. 1 2021.
• Citações Web of Science: 0

Resumo

Trypanosoma vivax infections cause nonspecific clinical signs in cattle associated with aparasitemic intervals, making disease diagnosis a challenge. In Brazil, diminazene aceturate and isometamidium chloride (ISM) are available to treat bovine trypanosomosis. The objective of this study was to follow-up, by molecular and serological techniques, dairy cattle naturally infected by T. vivax after ISM treatment. Thirty cattle naturally infected with T vivax received two applications of ISM, at a dosage of 1.0 mg/kg intramuscularly, on days 0 and 150. For T. vivax diagnosis, EDTA-blood and serum samples were evaluated on 0, 7, 15, 30, 60, 90, 120, 150, 180, 210, and 240 days after treatment PCR, Loop-mediated isothermal amplification (LAMP) and ELISA. Animals with persistent detection of T. vivax DNA by both PCR and LAMP were found and continuous detection of anti-T. vivax IgG antibodies by ELISA, suggesting the presence of T. vivax resistance to ISM. The combination of LAMP and ELISA tests can prevent misdiagnosis of the parasite clearance in treated cattle, contributing to better disease control. This is the first experiment that demonstrates the persistence infection of T. vivax under ISM treatment in a natural infected herd and evidence of ISM chemotherapy-resistant T. vivax in Brazil. (AU)

• Processo FAPESP: 15/25397-6 - Utilização da LAMP para diagnóstico e avaliação da eficácia terapêutica do cloridrato de isometamidium em bovinos naturalmente infectados com Trypanosoma vivax
• Beneficiário: Kayo José Garcia de Almeida Castilho Neto
• Modalidade de apoio: Bolsas no Brasil - Mestrado