The synergistic actions of hydrolytic genes reveal the mechanism of Trichoderma harzianum for cellulose degradation

Autor(es)

Almeida, Deborah Aires ^{[1, 2]} ; Crivelente Horta, Maria Augusta ^{[1, 2, 3]} ; Ferreira Filho, Jaire Alves ^{[1, 2]} ; Murad, Natalia Faraj ^[2] ; de Souza, Anete Pereira ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Grad Program Genet & Mol Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Ctr Mol Biol & Genet Engn CBMEG, Cidade Univ Zeferino Vaz, Ave Candido Rondon 400, BR-13083875 Campinas, SP - Brazil

^[3] Tech Univ Munich, Holzforsch Munchen, TUM Sch Life Sci Weihenstephan, D-85354 Freising Weihenstephan - Germany

^[4] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Cidade Univ Zeferino Vaz, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biotechnology; v. 334, p. 1-10, JUN 20 2021.
• Citações Web of Science: 0

Resumo

Bioprospecting genes and proteins related to plant biomass degradation is an attractive approach for the identification of target genes for biotechnological purposes, especially those with potential applications in the biorefinery industry that can enhance second-generation ethanol production technology. Trichoderma harzianum is a potential candidate for cellulolytic enzyme prospection and production. Herein, the enzymatic activities, transcriptome, exoproteome, and coexpression networks of the T. harzianum strain CBMAI-0179 were examined under biomass degradation conditions. We identified differentially expressed genes (DEGs) and carbohydrateactive enzyme (CAZyme) genes related to plant biomass degradation and compared them with those of strains from congeneric species (T. harzianum IOC-3844 and T. atroviride CBMAI-0020). T. harzianum CBMAI-0179 harbors strain- and treatment-specific CAZyme genes and transcription factors. We detected important proteins related to biomass degradation, including 13-glucosidases, endoglucanases, cellobiohydrolases, lytic polysaccharide monooxygenases, endo-1,4-13-xylanases and 13-mannanases. Based on coexpression networks, an enriched cluster with degradative enzymes was described, and the subnetwork of CAZymes revealed strong correlations among important secreted proteins and differentially expressed CAZyme genes. Our results provide valuable information for future studies on the genetic regulation of plant cell wall-degrading enzymes. This knowledge can be exploited for the improvement of enzymatic reactions in biomass degradation for bioethanol production. (AU)

• Processo FAPESP: 15/09202-0 - Estudo de regiões genômicas de Trichoderma harzianum associadas ao controle da expressão de enzimas envolvidas na degradação de biomassa
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular