Effects of Antifungal Carriers Based on Chitosan-Coated Iron Oxide Nanoparticles on Microcosm Biofilms

Autor(es)

Caldeirao, Anne Caroline Morais ^[1] ; Araujo, Heitor Ceolin ^[2] ; Tomasella, Camila Miranda ^[3] ; Sampaio, Caio ^[2] ; dos Santos Oliveira, Marcelo Jose ^[4] ; Ramage, Gordon ^[5] ; Pessan, Juliano Pelim ^[2] ; Monteiro, Douglas Roberto ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Western Sao Paulo UNOESTE, GPD Masters Degree, Grad Program Dent, BR-19050920 Presidente Prudente - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Dept Prevent & Restorat Dent, BR-16015050 Aracatuba - Brazil

^[3] Univ Western Sao Paulo UNOESTE, Sch Dent, BR-19050920 Presidente Prudente - Brazil

^[4] Sao Paulo State Univ Unesp, Sch Technol & Appl Sci FCT, Dept Phys, BR-19060900 Presidente Prudente - Brazil

^[5] Univ Glasgow, Coll Med Vet & Life Sci, Sch Med Dent & Nursing, Oral Sci Res Grp, Glasgow Dent Sch, Glasgow G2 3JZ, Lanark - Scotland

• Tipo de documento: Artigo Científico
• Fonte: ANTIBIOTICS-BASEL; v. 10, n. 5 MAY 2021.
• Citações Web of Science: 0

Resumo

Resistance of Candida species to conventional therapies has motivated the development of antifungal nanocarriers based on iron oxide nanoparticles (IONPs) coated with chitosan (CS). This study evaluates the effects of IONPs-CS as carriers of miconazole (MCZ) or fluconazole (FLZ) on microcosm biofilms. Pooled saliva from two healthy volunteers supplemented with C. albicans and C. glabrata was the inoculum for biofilm formation. Biofilms were formed for 96 h on coverslips using the Amsterdam Active Attachment model, followed by 24 h treatment with nanocarriers containing different concentrations of each antifungal (78 and 156 mu g/mL). MCZ or FLZ (156 mu g/mL), and untreated biofilms were considered as controls. Anti-biofilm effects were evaluated by enumeration of colony-forming units (CFUs), composition of the extracellular matrix, lactic acid production, and structure and live/dead biofilm cells (confocal laser scanning microscopy-CLSM). Data were analyzed by one-way ANOVA and Fisher LSD's test (alpha = 0.05). IONPs-CS carrying MCZ or FLZ were the most effective treatments in reducing CFUs compared to either an antifungal agent alone for C. albicans and MCZ for C. glabrata. Significant reductions in mutans streptococci and Lactobacillus spp. were shown, though mainly for the MCZ nanocarrier. Antifungals and their nanocarriers also showed significantly higher proportions of dead cells compared to untreated biofilm by CLSM (p < 0.001), and promoted significant reductions in lactic acid, while simultaneously showing increases in some components of the extracellular matrix. These findings reinforce the use of nanocarriers as effective alternatives to fight oral fungal infections. (AU)

• Processo FAPESP: 17/24416-2 - Síntese e efeito antibiofilme de dois novos nanosistemas carreadores de drogas antifúngicas
• Beneficiário: Douglas Roberto Monteiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular