Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis

Autor(es):Mostrar menos -

Barbosa, Patricia ^[1] ; Schemczssen-Graeff, Zelinda ^[2] ; Marques, Andre ^[3] ; da Silva, Maelin ^[2] ; Favero, Giovani Marino ^[4] ; Sobreiro, Bernardo Passos ^[5] ; de Almeida, Mara Cristina ^[2] ; Moreira-Filho, Orlando ^[1] ; Silva, Duilio Mazzoni Zerbinato de Andrade ^[6] ; Porto-Foresti, Fabio ^[7] ; Foresti, Fausto ^[6] ; Artoni, Roberto Ferreira ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolut, Post Grad Program Evolutionary Genet & Mol Biol, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Ponta Grossa, Dept Struct Mol & Genet Biol, Post Grad Program Evolutionary Biol, Ave Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, PR - Brazil

^[3] Univ Fed Pernambuco, Dept Bot, BR-50670901 Recife, PE - Brazil

^[4] Univ Estadual Ponta Grossa, Dept Gen Biol, Ave Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, PR - Brazil

^[5] Univ Estadual Ponta Grossa, Dept Med, Ave Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, PR - Brazil

^[6] Sao Paulo State Univ UNESP, Dept Struct & Funct Biol, Inst Biosci Botucatu, Botucatu, SP - Brazil

^[7] Sao Paulo State Univ UNESP, Fac Sci, BR-01049010 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 10, n. 5 MAY 2021.
• Citações Web of Science: 0

Resumo

The way in which transcriptional activity overcomes the physical DNA structure and gene regulation mechanisms involves complex processes that are not yet fully understood. Modifications in the cytosine-guanine sequence of DNA by 5-mC are preferentially located in heterochromatic regions and are related to gene silencing. Herein, we investigate evidence of epigenetic regulation related to the B chromosome model and transposable elements in A. scabripinnis. Indirect immunofluorescence using anti-5-mC to mark methylated regions was employed along with quantitative ELISA to determine the total genomic DNA methylation level. 5-mC signals were dispersed in the chromosomes of both females and males, with preferential accumulation in the B chromosome. In addition to the heterochromatic methylated regions, our results suggest that methylation is associated with transposable elements (LINE and Tc1-Mariner). Heterochromatin content was measured based on the C-band length in relation to the size of chromosome 1. The B chromosome in A. scabripinnis comprises heterochromatin located in the pericentromeric region of both arms of this isochromosome. In this context, individuals with B chromosomes should have an increased heterochromatin content when compared to individuals that do not. Although, both heterochromatin content and genome methylation showed no significant differences between sexes or in relation to the occurrence of B chromosomes. Our evidence suggests that the B chromosome can have a compensation effect on the heterochromatin content and that methylation possibly operates to silence TEs in A. scabripinnis. This represents a sui generis compensation and gene activity buffering mechanism. (AU)

• Processo FAPESP: 17/22447-8 - Atividade Gênica Comparada nos Cromossomos B de espécies de Astyanax (Teleostei, Characiformes)
• Beneficiário: Duílio Mazzoni Zerbinato de Andrade Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado