Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States

Texto completo
Autor(es):
Ames, Cheryl Lewis [1, 2, 3] ; Ohdera, Aki H. [4, 1] ; Colston, Sophie M. [2] ; Collins, Allen G. [1, 5] ; Fitt, William K. [6] ; Morandini, Andre C. [7, 8] ; Erickson, Jeffrey S. [9] ; Vora, Gary J. [9]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Smithsonian Inst, Dept Invertebrate Zool, Natl Museum Nat Hist, Washington, DC 20560 - USA
[2] US Naval Res Lab, Natl Res Council, Natl Acad Sci, Washington, DC 20375 - USA
[3] Tohoku Univ, Grad Sch Agr Sci, Fac Agr, Sendai, Miyagi - Japan
[4] CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 - USA
[5] Natl Ocean Atmospher Adm, Natl Systemat Lab, Fisheries Serv, Natl Museum Nat Hist, Smithsonian Inst, Washington, DC - USA
[6] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 - USA
[7] Univ Sao Paulo, Ctr Biol Marinha, Sao Sebastiao - Brazil
[8] Univ Sao Paulo, Inst Biociencias, Dept Zool, Sao Paulo - Brazil
[9] US Naval Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 - USA
Número total de Afiliações: 9
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MARINE SCIENCE; v. 8, APR 13 2021.
Citações Web of Science: 0
Resumo

Recent advances in molecular sequencing technology and the increased availability of fieldable laboratory equipment have provided researchers with the opportunity to conduct real-time or near real-time gene-based biodiversity assessments of aquatic ecosystems. In this study, we developed a workflow and portable kit for fieldable environmental DNA sequencing (FeDS) and tested its efficacy by characterizing the breadth of jellyfish (Medusozoa) taxa in the coastal waters of the Upper and Lower Florida Keys. Environmental DNA was isolated from seawater collection events at eight sites and samples were subjected to medusozoan 16S rRNA gene and metazoan mitochondrial cytochrome oxidase 1 gene profiling via metabarcoding onsite. In total, FeDS yielded 175,326 processed sequence reads providing evidence for 53 medusozoan taxa. Our most salient findings revealed eDNA from: (1) two venomous box jellyfish (Cubozoa) species, including taxa whose stings cause the notorious Irukandji envenomation syndrome; (2) two species of potentially introduced stalked jellyfish (Staurozoa); and (3) a likely cryptic species of upside-down jellyfish (Scyphozoa). Taken together, the results of this study highlight the merits of FeDS in conducting biodiversity surveys of endemic and introduced species, and as a potential tool for assessing envenomation and/or conservation-related threats. (AU)

Processo FAPESP: 15/21007-9 - Reconhecendo a diversidade de águas-vivas (Medusozoa, Rhopaliophora)
Beneficiário:André Carrara Morandini
Modalidade de apoio: Auxílio à Pesquisa - Regular