Curcuminoid-Tailored Interfacial Free Energy of Hydrophobic Fibers for Enhanced Biological Properties

Autor(es)

de Deus, Wevernilson F. ^[1] ; de Franca, Bruna M. ^[2] ; Forero, Josue Sebastian B. ^[2] ; Granato, Alessandro E. C. ^[3] ; Ulrich, Henning ^[3] ; Doria, Anelise C. O. C. ^[4] ; Amaral, Marcello M. ^[1] ; Slabon, Adam ^[5] ; Rodrigues, Bruno V. M. ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Brasil, Inst Cient & Tecnol, BR-08230030 Sao Paulo - Brazil

^[2] Univ Fed Rio de Janeiro, Inst Quim, BR-21941909 Rio De Janeiro - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil

^[4] Univ Vale Paraiba, Lab Biotecnol & Plasmas Eletr, IP&D, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[5] Stockholm Univ, Dept Mat & Environm Chem, S-10691 Stockholm - Sweden

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 13, n. 21, p. 24493-24504, JUN 2 2021.
• Citações Web of Science: 1

Resumo

The ability of mimicking the extracellular matrix architecture has gained electrospun scaffolds a prominent space into the tissue engineering field. The high surface-to-volume aspect ratio of nanofibers increases their bioactivity while enhancing the bonding strength with the host tissue. Over the years, numerous polyesters, such as poly(lactic acid) (PLA), have been consolidated as excellent matrices for biomedical applications. However, this class of polymers usually has a high hydrophobic character, which limits cell attachment and proliferation, and therefore decreases biological interactions. In this way, functionalization of polyester-based materials is often performed in order to modify their interfacial free energy and achieve more hydrophilic surfaces. Herein, we report the preparation, characterization, and in vitro assessment of electrospun PLA fibers with low contents (0.1 wt %) of different curcuminoids featuring pi-conjugated systems, and a central beta-diketone unit, including curcumin itself. We evaluated the potential of these materials for photochemical and biomedical purposes. For this, we investigated their optical properties, water contact angle, and surface features while assessing their in vitro behavior using SH-SY5Y cells. Our results demonstrate the successful generation of homogeneous and defect-free fluorescent fibers, which are noncytotoxic, exhibit enhanced hydrophilicity, and as such greater cell adhesion and proliferation toward neuroblastoma cells. The unexpected tailoring of the scaffolds' interfacial free energy has been associated with the strong interactions between the PLA hydrophobic sites and the nonpolar groups from curcuminoids, which indicate its role for releasing hydrophilic sites from both parts. This investigation reveals a straightforward approach to produce photoluminescent 3D-scaffolds with enhanced biological properties by using a polymer that is essentially hydrophobic combined with the low contents of photoactive and multifunctional curcuminoids. (AU)

• Processo FAPESP: 17/18826-3 - Materiais nanoestruturados sustentáveis baseados em polímeros biodegradáveis e nanopartículas de carbono luminescentes
• Beneficiário: Bruno Vinícius Manzolli Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/21851-0 - Desenvolvimento de metodologias de análise de imagens e sinais de tomografia por coerência óptica (OCT) para avaliação de angiogênese cutânea
• Beneficiário: Marcello Magri Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular