Subtypes of Blastocystis sp. isolated in fecal samples from transplant candidates in Sao Paulo, Brazil

Autor(es):Mostrar menos -

Silva, Maria do Rosario A. ^[1] ; Melo, Gessica B. ^[1] ; Malta, Fernanda M. ^[2] ; Abdala, Edson ^[3] ; Costa, Silvia F. ^[3] ; Pierrotti, Ligia C. ^[3] ; Goncalves, Elenice M. N. ^[3] ; Castilho, Vera L. P. ^[3] ; Chieffi, Pedro P. ^[4] ; Gryschek, Ronaldo C. B. ^[1] ; Paula, Fabiana M. ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med Univ Sao Paulo, Inst Med Trop, Lab Imunopatol Esquistossomose LIM 06, Sao Paulo - Brazil

^[2] Fac Med Univ Sao Paulo, Inst Med Trop, Lab Gastroenterol & Hepatol Trop LIM 07, Sao Paulo - Brazil

^[3] Fac Med Univ Sao Paulo, Hosp Clin, Sao Paulo - Brazil

^[4] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITE EPIDEMIOLOGY AND CONTROL; v. 8, FEB 2020.
• Citações Web of Science: 3

Resumo

Blastocystis sp. is an intestinal protozoan commonly found in fecal samples of many animal species, including humans, but poorly studied in transplant candidates. The aim of this study was to evaluate the occurrence and molecular identification of Blastocystis sp. in fecal samples from transplant candidates. A polymerase chain reaction was performed using specific primers for Blastocystis ribosomal DNA. The DNA sequences obtained were aligned and compared with other sequences from the GenBank and MLST databases. The analyzed samples showed a positivity of 16% (24 of 150) for Blastocystis sp. The highest occurrence was observed in renal transplant candidates (31.4%), followed by hepatic transplant candidates (10.4%) and candidates for bone marrow transplantation (5.9%). Subtype (ST) 3 (45.8%) was the most prevalent among the isolates, followed by ST1 (375%), ST2 (12.5%), and ST7 (4.2%). This is the first study of molecular identification Blastocystis sp. in transplant candidates. Our results confirmed that ST3 was the most common subtype in transplant candidates and reinforce the importance of new studies to investigate of Blastocystis sp. in these patients. (C) 2019 Published by Elsevier Ltd on behalf of World Federation of Parasitologists. (AU)

• Processo FAPESP: 15/18213-6 - Identificação de genótipos de Blastocystis spp. em amostras de fezes e sua relação com urticária crônica
• Beneficiário: Ronaldo Cesar Borges Cryschek
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51110-2 - Investigação de técnicas diagnósticas para identificação da infecção por S. stercoralis em pacientes com diferentes formas de imunodepressão
• Beneficiário: Pedro Paulo Chieffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular