Residual biomass from surfactin production is a source of arginase and adsorbed surfactin that is useful for environmental remediation

Autor(es):Mostrar menos -

de Carvalho Silveira, Thais ^[1] ; Gomes, Wyllerson Evaristo ^[2] ; Tonon, Giovana Chinaglia ^[3] ; Beatto, Thaina Godoy ^{[3, 2]} ; Spogis, Nicolas ^[3] ; Cunha, Luiz Henrique Dallan ^[3] ; Lattaro, Bruno Pera ^[3] ; Nogueira, Alessandra Borin ^[3] ; Mendes, Renata Kelly ^{[3, 2]} ; Alvarenga, Danillo Oliveira ^[4] ; Etchegaray, Augusto ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Pontificia Univ Catolica Campinas, Ctr Ciencias Vida, Programa Posgrad Ciencias Saude, Campinas, SP - Brazil

^[2] Pontificia Univ Catolica Campinas, Programa Posgrad Sistemas Infraestrutura Urbana, Ctr Ciencias Ambientais & Tecnol, Campinas, SP - Brazil

^[3] Pontificia Univ Catolica Campinas PUC Campinas, Fac Quim, Ctr Ciencias Ambientais & Tecnol, Rua Prof Doutor Euryclides de Jesus Zerbini 1-516, BR-13087571 Campinas, SP - Brazil

^[4] Univ Copenhagen, Dept Biol, Univ Pk 15, DK-2100 Copenhagen - Denmark

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 37, n. 7 JUL 2021.
• Citações Web of Science: 1

Resumo

Lipopeptides are important secondary metabolites produced by microbes. They find applications in environmental decontamination and in the chemical, pharmaceutical and food industries. However, their production is expensive. In the present work we propose three strategies to lower the production costs of surfactin. First, the coproduction of surfactin and arginase in a single growth. Second, extract the fraction of surfactin that adsorbs to the biomass and is removed from the growth medium through centrifugation. Third, use microbial biomass for the remediation of organic and inorganic contaminants. The coproduction of surfactin and arginase was evaluated by factorial design experiments using the LB medium supplemented with arginine. The best conditions for surfactin production were 22 h of growth at 37 degrees C using LB supplemented with arginine 7.3 g/L. Almost similar conditions were found to produce highest levels of arginase, 24 h and 6.45 g/L arginine. Decontamination of phenol and copper from artificial samples was attained by treatment with residues from lipopeptide production. Thus, cell suspensions and wash-waters used to extract surfactin from the biomass. Cell suspensions were used to successfully remove hydroquinone. Cell suspensions and wash-waters containing surfactin were successfully used to recover copper from solution. Specific monitoring methods were used for phenol and metal solutions, respectively a biosensor based on tyrosinase and either atomic absorption flame ionization spectrometry or absorbance coupled to the Arduino (TM) platform. Therefore, we report three alternative strategies to lower the production costs in lipopeptide production, which include the effective recovery of copper and phenol from contaminated waters using residues from surfactin production. Graphic abstract Sustainable and profitable production of surfactin can be achieved by a coproduction strategy of lipopeptides and enzymes. Lipopeptides are collected in the supernatant and enzymes in the biomass. In addition, lipopeptides that coprecipitate with biomass can be recovered by washing. Lipopeptide wash-waters find applications in remediation and cells can also be used for environmental decontamination. {[}GRAPHICS] . (AU)

• Processo FAPESP: 13/20570-6 - Produção sustentável de biossurfactantes com aplicações para medicina e remediação ambiental
• Beneficiário: Augusto Etchegaray Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular