Chemical and morphological effects of the contraceptive hormone 17 alpha-ethynylestradiol on fluid lipid membranes

Autor(es)

Marques Ruiz, Gilia Cristine ^[1] ; do Carmo Morato, Luis Fernando ^[1] ; Pazin, Wallance Moreira ^[1] ; Milano, Francesco ^[2] ; Leopoldo Constantino, Carlos Jose ^[1] ; Valli, Ludovico ^{[3, 4]} ; Giotta, Livia ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Technol & Appl Sci, Dept Phys, Presidente Prudente, SP - Brazil

^[2] Italian Natl Res Council CNR ISPA, Inst Sci Food Prod, I-73100 Lecce - Italy

^[3] Univ Salento, Dept Biol & Environm Sci & Technol DiSTeBA, I-73100 Lecce - Italy

^[4] Consorzio Interuniv Nazl Sci & Tecnol Mat INSTM, Unita Lecce, I-73100 Lecce - Italy

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 204, AUG 2021.
• Citações Web of Science: 0

Resumo

The lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the interaction (causes and consequences) of the hormone 17 alpha-ethinylestradiol (EE2) with lipid monolayers (prepared as Langmuir films) and bilayers prepared as small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs). Both fluidity and majority chemical composition of real plasma cell membrane were guaranteed using the phospholipid 1-palmitoil-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC). Surface pressure-mean molecular area (pi-A) isotherms and PM-IRRAS measurements highlighted the strong interaction of EE2 with POPC monolayers, leading the hormone to remain at the air/water interface and promoting its penetration into the phospholipid hydrophobic chains. In the case of bilayers, the entrance of the hormone inside the SUV is likely facilitated by their high curvature. In GUVs, EE2 was responsible for changes in the spherical shape, forming structures like buds and lipid protrusions. The set of results indicates the strong effects of EE2 on fluid membranes, which is an important feature to predict its damage in human cells. (AU)

• Processo FAPESP: 16/09633-4 - Mecanismo de ação do composto bioativo Artepillin C em sistemas miméticos de membranas e membranas citoplasmáticas de células tumorais
• Beneficiário: Wallance Moreira Pazin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado