Molecular Characterization of Xyloglucanase cel74a from Trichoderma reesei

Autor(es)

Lopes, Douglas Christian Borges ^[1] ; Carraro, Claudia Batista ^[1] ; Silva, Roberto Nascimento ^[1] ; de Paula, Renato Graciano ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Dept Biochem & Immunol, Mol Biotechnol Lab, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Physiol Sci, BR-29047105 Vitoria, ES - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 9 MAY 2021.
• Citações Web of Science: 0

Resumo

Background: The filamentous fungus Trichoderma reesei is used on an industrial scale to produce enzymes of biotechnological interest. This fungus has a complex cellulolytic system involved in the degradation of lignocellulosic biomass. However, several aspects related to the regulation of the expression of holocellulolytic genes and the production of cellulases by this fungus are still understood. Methods: Here, we constructed a null mutant strain for the xyloglucanase cel74a gene and performed the characterization of the Delta cel74a strain to evaluate the genetic regulation of the holocellulases during sugarcane bagasse (SCB) cultivation. Results: Our results demonstrate that the deletion of xyloglucanase cel74a may impact the regulation of holocellulase expression during SCB cultivation. The expression of cellulases cel7a, cel7b, and cel6a was reduced in Delta cel74a strain, while the hemicellulases xyn1 and xyn2 were increased in the presence of SCB. The cel74a mutation also affected the xyloglucan hydrolysis patterns. In addition, CEL74A activity was modulated in the presence of calcium, suggesting that this ion may be required for efficient degradation of xyloglucan. Conclusions: CEL74A affects the regulation of holocellulolytic genes and the efficient degradation of SCB in T. reesei. This data makes a significant contribution to our understanding of the carbon utilization of fungal strains as a whole. (AU)

• Processo FAPESP: 16/23233-9 - Caracterização da via de sinalização dependente de cálcio na regulação da expressão e secreção de celulases pelo fungo Trichoderma reesei (Hypocrea jecorina)
• Beneficiário: Renato Graciano de Paula
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/09708-4 - Expressão das enzimas acessórias polissacarídeo mono-oxigenase e celobiose desidrogenase de Aspergillus nidulans em Trichoderma reesei e avaliação da eficiência da degradação do bagaço de cana de açúcar
• Beneficiário: Douglas Christian Borges Lopes
• Modalidade de apoio: Bolsas no Brasil - Mestrado