Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems

Autor(es)

Checchio, Mirela Vantini ^[1] ; de Cassia Alves, Rita ^[2] ; de Oliveira, Kevein Ruas ^[1] ; Moro, Gustavo Vitti ^[3] ; Santos, Durvalina Maria Mathias dos ^[1] ; Gratao, Priscila Lupino ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, BR-14884900 Jaboticabal, SP - Brazil

^[2] Inst Nacl Semiarido INSA, Nucleo Prod Vegetal, BR-58434700 Campina Grande, PB - Brazil

^[3] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Prod Vegetal, BR-14884900 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PLANT RESEARCH; v. 134, n. 6 JUL 2021.
• Citações Web of Science: 0

Resumo

Salinity has become one of the major factors limiting agricultural production. In this regard, different cost-effective management strategies such as the use of plant growth-promoting bacteria (PGPB) as inoculants to alleviate salt-stress conditions and minimize plant productivity losses have been used in agricultural systems. The aim of this study was to characterize induced antioxidant responses in corn through inoculation with Azospirillum brasilense and examine the relationship between these responses and the acquired salt-stress tolerance. Treatments were performed by combining sodium chloride (0 and 100 mM NaCl) through irrigation water with absence and presence of A. brasilense inoculation. The experiment was performed in a completely randomized design with four replications. Lipid peroxidation (malondialdehyde {[}MDA]), and nitrogen (N), sodium (Na+) and potassium (K+) contents, as well as dry biomass, glycine betaine, and antioxidant enzymes activities such as of superoxide dismutase (SOD, EC 1. 15. 1. 1), glutathione reductase (GR, EC 1. 6. 4. 2), guaiacol peroxidase (GPOX, EC 1. 11. 1. 7), and glutathione peroxidase (GSH-PX, EC 1. 11. 1. 9) were determined. Overall results indicated that plants treated with 100 mM NaCl showed the most pronounced salt-stress damages with consequent increase in MDA content. However, inoculated plants showed an enhanced capacity to withstand or avoid salt-stress damages. These results could be attributed, at least in part, to the increased activity of antioxidant enzymes. Our results suggest that A. brasilense may confer tolerance to salt stress in corn plants enhancing antioxidant responses, primarily by the enzymes GSH-PX and GPOX, and the osmolyte glycine betaine. (AU)

• Processo FAPESP: 17/04787-6 - O selênio na modulação da síntese de etileno e tolerância ao estresse induzido por cádmio
• Beneficiário: Priscila Lupino Gratão
• Modalidade de apoio: Auxílio à Pesquisa - Regular