Exposure to sugarcane borer-induced plant volatile (E)-caryophyllene enhances parasitoid recruitment

Autor(es):Mostrar menos -

Riffel, Alessandro ^[1] ; Filho, Benisio F. Silva ^[2] ; dos Santos, Sanielly P. A. ^[3] ; da Silva, Wbyratan L. ^[3] ; Ribeiro, Thyago F. L. ^[2] ; Oliveira, Demetrios J. A. ^[2] ; de Oliveira, Jaim Simoes ^{[3, 4]} ; da Costa, Joao Gomes ^[1] ; Caulfield, John C. ^[5] ; Goulart, Henrique F. ^[3] ; Santana, Antonio E. G. ^[3] ; Birkett, Michael A. ^[5]

Afiliação do(s) autor(es)

^[1] Embrapa Tabuleiros Costeiros, Unidade Pesquisa Rio Largo, Rio Largo, AL - Brazil

^[2] Univ Fed Alagoas UFAL, Inst Quim & Biotecnol, Maceio, Alagoas - Brazil

^[3] Univ Fed Alagoas UFAL, Lab Pesquisa Recursos Nat LPqRN, Campus Engn & Ciencias Agr CECA, Maceio, Alagoas - Brazil

^[4] Ctr Univ Tiradentes UNIT AL, Av Comendador Gustavo Paiva 5017, BR-57038000 Maceio, Alagoas - Brazil

^[5] Rothamsted Res, Biointeract & Crop Protect Dept, Harpenden AL5 2JQ, Herts - England

• Tipo de documento: Artigo Científico
• Fonte: Entomologia Experimentalis et Applicata; v. 169, n. 10 JUL 2021.
• Citações Web of Science: 0

Resumo

Predators and parasitoids locate herbivore-infested plants via detection of herbivore-induced plant volatiles (HIPVs) that are released in response to pest damage. Synthetic HIPVs have been proposed as tools to enhance the biological control of crop pests. The sugarcane borer, Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae), is a key herbivore pest of sugarcane, Saccharum spp. (Poaceae), in the Americas. To manage D. saccharalis in Brazil, more than 3 million ha of sugarcane have been treated with the larval parasitoid Cotesia flavipes (Cameron) (Hymenoptera: Braconidae). In this study, the role of sugarcane HIPVs as cues in the host-finding process of C. flavipes was investigated using a combination of dynamic headspace collection, chemical analysis, and laboratory behavior experiments. Comparison of volatile organic compounds (VOCs) collected from D. saccharalis-damaged and healthy sugarcane revealed very similar profiles, apart from significantly higher levels of the sesquiterpene (E)-caryophyllene released from damaged plants. Naive female C. flavipes spent significantly more time in the olfactometer arm containing VOCs from D. saccharalis-damaged plants but showed no preference for VOCs from healthy plants. When (E)-caryophyllene was added to VOCs from healthy plants, parasitoids spent more time in the arm containing the combined treatment. Furthermore, in a dose-response experiment with synthetic (E)-caryophyllene, naive parasitoids preferred the compound across a dose range of 3-300 ng, and experienced parasitoids - preexposed to (E)-caryophyllene - responded to doses of (E)-caryophyllene as low as 0.03 ng. These results suggest that C. flavipes can use (E)-caryophyllene as a cue to locate D. saccharalis-infested sugarcane plants. Moreover, experienced females appear to respond to lower doses than naive females. These results potentially pave the way for increasing the efficiency of C. flavipes in biological control of D. saccharalis, the most important pest of sugarcane and maize crops in the Western Hemisphere, and also a major pest of rice and sorghum crops. (AU)

• Processo FAPESP: 14/50871-0 - INCT 2014: Instituto Nacional de Ciência Tecnologia de Semioquímicos na Agricultura
• Beneficiário: José Roberto Postali Parra
• Modalidade de apoio: Auxílio à Pesquisa - Temático