Genotoxic and melanic alterations in Lithobates catesbeianus (anura) tadpoles exposed to fipronil insecticide

Autor(es)

Santos, Arleto T. ^[1] ; Valverde, Bruno S. L. ^[1] ; De Oliveira, Classius ^[2] ; Franco-Belussi, Lilian ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Programa Posgrad Biodiversidade, Campus Sao Jose Rio Preto, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Paulo - Brazil

^[3] Univ Fed Mato Grosso do Sul, Lab Patol Expt, Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 28, n. 16, p. 20072-20081, APR 2021.
• Citações Web of Science: 0

Resumo

The aim of this study was to evaluate the genotoxic and morphological systemic effects of both an acute and a chronic exposure of bullfrog tadpoles to fipronil. Lithobates catesbeianus tadpoles had morphological biomarkers (skin, liver, and blood) analyzed at Gosner stages 36-38, when exposed to four different concentrations of Regent (R) 800 WG (80% fipronil): 0.00 (control), 0.04, 0.08, 0.4 mg/L, and four experimental times: 4, 8, 12, and 16 days. Body darkness responded directly to the treatment and exposure time. There was a treatment-dependent decrease in darkness of heads and tails. In relation to the biometric analysis, fipronil induced a decrease in the individual weight and liver mass at the end of the experiments, whereas the hepatosomatic index did not vary according to the treatment. For the exposed animals and for the control group, the area of hepatic melanin increased as exposure time increased. Fipronil has genotoxic effects on L. catesbeianus tadpoles even after short exposure times (e.g., 4 and 8 days), and the main nuclear abnormality is in the anucleate cells. A relevant correlation was observed between genotoxic biomarkers and cutaneous and internal melanin. The frequency of nuclear abnormalities is inversely correlated both with the hepatic melanin area and with the cutaneous melanin of animals. Fipronil has distinct systemic effects on tadpoles based on its concentration, as well as on its exposure time. Such alterations (pigmentation level and rate of erythrocyte abnormality) result in morphological and physiological effects, which may compromise the behavior and survival of the anurans. (AU)

• Processo FAPESP: 18/01078-7 - O fígado de anfíbios anuros: morfologia comparada entre 10 espécies e ação do benzopireno nos melanomacrófagos de Physalaemus cuvieri
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular