Microsatellites for the Neotropical ant, Camponotus leydigi (Hymenoptera: Formicidae)

Autor(es)

Goncalves-Neto, Salatiel ^[1] ; Azevedo-Silva, Marianne ^[2] ; Lemos, Alessandra S. M. ^[1] ; Souza, Anete P. ^[3] ; Oliveira, Paulo S. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Biol Anim, Grad Ciencias Biol, Campinas - Brazil

^[2] Univ Estadual Campinas, Dept Biol Anim, Programa Posgrad Ecol, Campinas - Brazil

^[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Dept Biol Vegetal, Campinas - Brazil

^[4] Univ Estadual Campinas, Dept Biol Anim, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENTOMOLOGICAL SCIENCE; v. 24, n. 1, p. 79-84, MAR 2021.
• Citações Web of Science: 0

Resumo

Ants (Hymenoptera: Formicidae) are dominant social insects that play important ecological roles in terrestrial ecosystems. Camponotus leydigi (Forel) is widely distributed in the Neotropical region and is frequently found in the Brazilian cerrado savanna interacting with plants and other insects. Field observations indicate that C. leydigi has a polydomous nesting habit, but little is known about the genetic relationship among workers. In this study, we identify the first nine microsatellite loci for C. leydigi that will allow further investigation on its genetic diversity. We used a microsatellite-enriched library method. According to this method, repetitive sequences are captured with (CT)(8) and (GT)(8) biotin-linked probes, with subsequent recovery by streptavidin magnetic-coated beads. We observed that eight loci were polymorphic. The mean (+/- standard error) observed and expected heterozygosities were 0.55 +/- 0.23 and 0.73 +/- 0.28, respectively. The rarified allelic richness ranged from 1 to 5.32. The polymorphism contents were similar to diversity estimates found in markers previously developed for other Camponotus ants. These markers will be useful for future studies on population genetics and ecology of Camponotus ants in cerrado, including nesting ecology, colony structure, dispersal and conservation. (AU)

• Processo FAPESP: 17/18291-2 - Biodiversidade e ecologia molecular de formigas (Hymenoptera: Formicidae) ao longo de um gradiente latitudinal de Cerrado
• Beneficiário: Marianne Azevedo Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/12683-1 - O uso de marcadores moleculares na investigação de polidomia em uma formiga do Cerrado, Camponotus leydigi (Formicidae: Formicinae)
• Beneficiário: Salatiel Gonçalves Neto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/16645-1 - Ecologia molecular de formigas neotropicais
• Beneficiário: Paulo Sergio Moreira Carvalho de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 14/23141-1 - Ecologia de interações, ecologia comportamental e biologia molecular de formigas neotropicais
• Beneficiário: Paulo Sergio Moreira Carvalho de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular