An efficient protocol for in vitro propagation of Trichilia pallida Swartz (Meliaceae): a potential tree for natural insecticide production

Autor(es)

de Souza Prim, Pamela ^[1] ; de Oliveira, Leandro Francisco ^[2] ; Ribas, Luciana Lopes Fortes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Parana, Dept Bot, Av Cel Francisco H dos Santos, BR-81530000 Curitiba, PR - Brazil

^[2] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Rua Matao 277, Sala 107, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT CELL TISSUE AND ORGAN CULTURE; v. 144, n. 2, p. 469-476, FEB 2021.
• Citações Web of Science: 0

Resumo

Key message A complete protocol for germination and micropropagation of Trichilia pallida was established, a species with great potential for conservation and commercial purposes. In vitro and ex vitro germination was successfully achieved for seedling production from monoembryonic and polyembryonic seeds. The seedlings were used as a source of explants for establishing a micropropagation protocol of Trichilia pallida. Cotyledonary node and apical shoot sections were cultured in MS medium supplemented with 6-benzyladenine (BA; 0, 2.0, 4.0, or 8.0 mu M) during three subcultures in order to induce new shoots. Polyamines (PAs) were tested to promote shoot elongation and rooting. MS/2 medium supplemented with indole-3-butyric acid (IBA; 0, 1.25, 2.5, 5.0, and 10.0 mu M) was used for root induction. The results indicated better responses of shoot regeneration when adding 2.0 mu M BA to the culture medium, which promoted shoot regeneration (98% of explants and 5 to 7 shoots per explant), irrespective of the type of explant tested, during three subcultures. PAs showed a positive effect to promote shoot elongation with the supplementation of 0.5 mM spermidine (70%) and rooting of shoots with 2.0 mM putrescine (82%). Rooting was also achieved on MS/2 medium supplemented with 5.0 mu M IBA, where 84% of explants showed root development. The commercial substrates tested in combination with vermiculite (1:1 v/v) were efficient for acclimatization, allowing 87.76% and 75.52% of survival rate after 90 and 120 days, respectively, in a greenhouse. In conclusion, a feasible and complete in vitro propagation protocol from seedlings was developed for T. pallida. (AU)

• Processo FAPESP: 18/08215-0 - Estudo dos processos de regulação gênica pós-transcricional na embriogênese somática em Araucaria angustifolia
• Beneficiário: Leandro Francisco de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado