Benefits of Sebastiania hispida (Euphorbiaceae) extract and photobiomodulation therapy as potentially adjunctive strategies to be explored against snake envenoming

Autor(es)

Dourado, Doroty Mesquita ^{[1, 2, 3]} ; Matias, Rosemary ^[4] ; Kato da Silva, Baldomero Antonio ^[5] ; Milanesi, Fiorela Faria ^[6] ; Martello, Mayra Duarte ^[7] ; Marques dos Santos, Carlos Henrique ^[2] ; Lima Cardoso, Claudia Andrea ^[8] ; Vieira, Willians Fernando ^[1] ; Da Cruz-Hofling, Maria Alice ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

^[2] Anhanguera Uniderp Univ, Lab Toxicol & Med Plants, Campo Grande, MS - Brazil

^[3] Fed Univ Mato Grosso UFMS, Postgrad Program Infect & Parasit Dis, Campo Grande, MS - Brazil

^[4] Anhanguera Uniderp Univ, Postgrad Program Environm & Reg Dev, Campo Grande, MS - Brazil

^[5] Fed Univ Delta Parnaiba UFDPar, Postgrad Program Biomed Sci, Av Sao Sebastiao 2819, BR-64202020 Parnaiba, PI - Brazil

^[6] Oswald Cruz Fdn MS, Hlth & Environm Biodivers Program, Campo Grande, MS - Brazil

^[7] Fed Univ Mato Grosso UFMS, Postgrad Program Biochem & Mol Biol, Campo Grande, MS - Brazil

^[8] Mato Grosso State Univ UEMS, Dept Chem, Dourados, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 20, n. 8 AUG 2021.
• Citações Web of Science: 0

Resumo

The purpose of this study was to assess the topic use of Sebastiania hispida extract and low-level gallium-arsenide laser irradiation (GaAs, 904 nm) to reduce the local myonecrosis and edema of Bothrops moojeni snake venom-injected gastrocnemius. Wistar rats receiving intramuscular venom injection (VBm) were compared with saline control (S) and envenomed rats receiving local exposure to plant extract (VExt) or laser irradiation (VL). The phytochemistry and thin-layer chromatography of S. hispida extract indicated the presence of phenolic compounds like gallic acid and flavonoids including quercetin. Gastrocnemius of VExt and VL groups had a significant reduction of edema and creatine kinase (CK) activities and a greater Myogenin (MyoG) expression compared to VBm group, with the plant extract efficacy better than laser exposure. Reduction of edema and serum CK activities reflects a lessening of muscle damage, whereas the increase of MyoG indicates myoblast differentiation and acceleration of muscle repair. The S. hispida richness in phenolic compounds and flavonoids, such as the light modulatory ability to triggering a multitude of cell signalings likely underlie the positive outcomes. Our findings suggest both treatments as potential auxiliary tools to be explored in clinical trials in combination with anti-venom therapy after Bothropic snakebites. (AU)

• Processo FAPESP: 05/53625-1 - Ação dos mastoparanos e da fosfolipase A2 isolados do veneno de Polybia paulista (Hymenoptera, Epiponini) nos mecanismos de apoptose e necrose de células musculares e mitocôndrias isoladas de camundongos Balb/c
• Beneficiário: Maria Alice da Cruz Hofling
• Modalidade de apoio: Auxílio à Pesquisa - Regular