olecular organization in hydroperoxidized POPC bilayer

Autor(es)

Junqueira, Helena ^{[1, 2]} ; Schroder, Andre P. ^{[3, 4]} ; Thalmann, Fabrice ^[3] ; Klymchenko, Andrey ^[5] ; Mely, Yves ^[5] ; Baptista, Mauricio S. ^[1] ; Marques, Carlos M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Fis, Dept Fis Aplicada, CP 66318, BR-05314970 Sao Paulo - Brazil

^[3] Univ Strasbourg, CNRS, Inst Charles Sadron UPR22, F-67000 Strasbourg - France

^[4] Univ Lyon, INSA Lyon, CNRS UMR5259, LaMCoS, F-69621 Villeurbanne - France

^[5] Univ Strasbourg, Lab Bioimagerie & Pathol, UMR 7021 CNRS, Fac Pharm, 74 Route Rhin, CS 60024, F-67401 Illkirch Graffenstaden - France

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1863, n. 10 OCT 2021.
• Citações Web of Science: 0

Resumo

Lipid hydroperoxides are the primary reaction products of lipid oxidation, a natural outcome of life under oxygen. While playing a major role in cell metabolism, the microscopic origins of the effects of lipid hydroperoxidation on biomembranes remain elusive. Here we probe the polar structure of partially to fully hydroperoxidized bilayers of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) by a combination of environment-sensitive fluorescent probes and coarse-grained Martini numerical simulations. We find that the inserted organic hydroperoxide group -OOH migrates preferentially to the surface for bilayers with small fractions of hydroperoxidized lipids, but populates also significantly the bilayer interior for larger fractions. Our findings suggest that by modifying the intimate polarity of biomembranes, lipid peroxidation will have a significant impact on the activity of transmembrane proteins and on the bio-medical efficiency of membrane active molecules such as cell-penetrating and antimicrobial peptides. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/11640-0 - Relação entre o dano fotoinduzido em lipídeos e a permeabilização de membranas
• Beneficiário: Isabel de Oliveira Lima Bacellar
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto