erivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinon

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Virgens, Anangelica R. ^{[1, 2]} ; Goes, Heliana F. O. ^{[1, 2]} ; de Carvalho, Gabriel C. ^[2] ; Pietrobon, Anna Julia ^[2] ; C. C. Branco, Anna Claudia ^[2] ; Ramos, Yasmim A. L. ^[3] ; Pereira, Naiura V. ^[1] ; Orfali, Raquel L. ^[1] ; Aoki, Valeria ^[1] ; da Silva, Luiz Fernando F. ^[3] ; Sotto, Mirian N. ^[3] ; dos Reis, Vitor M. S. ^[1] ; Sato, Maria N. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Dermatol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Trop Med Inst Sao Paulo, Dept Dermatol, Lab Invest Med, LIM 56, LIM-56, Av Dr Eneas Carvalho Aguiar 470, 3rd Floor, BR-05403000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EXPERIMENTAL DERMATOLOGY; v. 31, n. 2 AUG 2021.
• Citações Web of Science: 0

Resumo

Background Methylisothiazolinone (MI) and Methylchloroisothiazolinone (MCI) are among the most common skin sensitizers, yet the immunological events that occur during MCI/MI allergic contact dermatitis (ACD) are still poorly understood. Objectives: To analyse dendrocytes, macrophage subtypes and T cells in skin during the elicitation phase of MCI/MI ACD. Methods Thirteen patients with positive patch test reactions to MCI/MI (ACD group) and 11 individuals with negative patch test results were selected. Skin biopsies were only performed at 48 hours of patch testing. Immunohistochemistry was conducted to assess T cells, dendrocytes (Factor XIIIa), M1 (p-Stat1, CD68) and M2 (c-Maf, CD163) macrophages. Transcriptional analyses were performed for cytokines and related factors, and further compared to atopic dermatitis samples (n=4). Immunofluorescence assays addressed T cells location, along with IL-4 or IL-13, within the skin. Results MCI/MI elicited dermal dendrocytes and macrophages, pronouncedly the M2 subtype. T cells, majorly CD4+ T cells, accumulated in the perivascular areas. Similarly, abundant IL-4 protein was detected in these areas. There was an upregulation of IL-4 and IL-13 mRNA expression, a mild increase in IFNG mRNA levels and a down-regulation of RORC in the ACD group. Immunofluorescence revealed dermal clusters of T cells co-localized with IL-4. Conclusions M2 macrophages and Th2 cells participate in the immunopathogenesis of MCI/MI ACD. Dermal dendrocytes and M2 macrophages may assist the formation of CD4+ T cells perivascular clusters. These findings render a mechanistic insight into the MCI/MI reaction. Further analysis at different timepoints of patch testing is required to fully comprehend this ACD kinetics. (AU)

• Processo FAPESP: 17/12475-4 - Análise dos receptores de reconhecimento de padrões e interação de células dendríticas-linfócitos T e macrófagos na dermatite de contato alérgica por metilisotiazolinona e metilcloroisotiazolinona
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• Modalidade de apoio: Auxílio à Pesquisa - Regular