A rheological approach to identify efficient biopolymer producing bacteria

Autor(es)

Malvar, Sara ^[1] ; Cardoso, Leticia O. B. ^{[2, 3]} ; Karolski, Bruno ^[3] ; Perpetuo, Elen A. ^{[4, 3]} ; Carmo, Bruno S. ^[1] ; Meneghini, Julio R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Mech Engn, Escola Politecn, Av Prof Mello Moraes 2231, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Interunit Program Biotechnol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Environm Res & Educ Ctr CEPEMA, Escola Politecn, Cubatao, SP - Brazil

^[4] Univ Fed Sao Paulo, Inst Marine Sci, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biotechnology and Bioengineering; v. 118, n. 2, p. 622-632, FEB 2021.
• Citações Web of Science: 0

Resumo

This study investigates the relationship between collective motion and propulsion of bacterial consortia and their biopolymer production efficiency. Rheological tests were conducted for suspensions of two different methanotrophic bacterial consortia obtained after enrichment of sediment samples from mangrove sites in Brazil. We considered the linear viscoelasticity region and analyzed the values of storage and loss moduli as functions of days of cultivation, for different values of the volume fraction. The suspensions' rheological behaviors reflected the bacterial growth stage. We found that the formation of structures over time in some types of consortia can hinder the movement of bacteria in the search for nutrients. The change in complex viscosity of the two consortia followed a different and rich behavior that appears to be closely related to their capacity to capture methane. Our analysis showed a possible correlation between collective motion, viscosity reduction, and biopolymer production. The pieces of evidence from this study suggest that the efficiency of bacterial motion is directly related to biopolymer production, and this could facilitate the process of identifying the best consortium of biopolymer producing bacteria. (AU)

• Processo FAPESP: 19/15754-7 - Estudo reológico de suspensões de microorganismos produtores de biopolímeros
• Beneficiário: Sara Malvar Mauá
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/14337-5 - Hidrodinâmica de Fluidos Ativos em Microescoamentos
• Beneficiário: Sara Malvar Mauá
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/22790-7 - Avaliação do potencial da produção de biopolímeros por bactérias metilotróficas
• Beneficiário: Elen Aquino Perpetuo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 14/50279-4 - Brasil Research Centre for Gas Innovation
• Beneficiário: Julio Romano Meneghini
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia