Pathogenic potential of Enterococcus faecalis strains isolated from root canals after unsuccessful endodontic treatment

Autor(es)

Francisco, Priscila Amanda ^[1] ; Fagundes, Pedro Ivo da Graca ^[1] ; Lemes-Junior, Joao Carlos ^[1] ; Lima, Augusto Rodrigues ^[1] ; Passini, Maicon Ricardo Zieberg ^[1] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Restorat Dent, Div Endodont, Piracicaba Dent Sch, Ave Limeira 901, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 9, p. 5171-5179, SEP 2021.
• Citações Web of Science: 1

Resumo

Objectives To evaluate strains of Enterococcus faecalis isolated from endodontic failures cases for (a) presence of virulence genes, namely, gelatinase production (gelE), surface protein (esp), collagen-binding adhesin (ace), cytolysin activator (cylA), E. faecalis antigen A (efaA) and aggregation substance (asa), all by using PCR; (b) biofilm formation capacity; and (c) activity of gelatinase and beta-lactamase. Materials and methods Twenty-five strains of E. faecalis were tested. The DNA extracted from these strains was used for identification of virulence genes by PCR and 1% agarose gel. Biofilm formation was performed on polystyrene microplates by using the violet crystal staining method. For assessment of the gelatinase activity, inoculum of pure cultures was deposited in tubes containing gelatin and a nutrient broth, whereas nitrocefin disks were used to assess the beta-lactamase action. Results The virulence genes efaA and cylA were detected in 100% of the strains, whereas gelE was present in 84%, ace in 68%, esp in 56% and asa in 48%. Four strains had no biofilm formation, 17 had poor formation and four had moderate formation. Gelatinase production was observed in three strains and beta-lactamase resistance in five strains of E. faecalis. Topic Diverse patterns of virulence gene detection were observed among the E. faecalis strains, with predominance of those capable of forming biofilm. A few strains have been found to hydrolyze gelatin proteins, whereas beta-lactamase resistance was detected in different isolates. (AU)

• Processo FAPESP: 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/00587-5 - Investigação dos genes de virulência de Enterococcus faecalis isolados de dentes associados ao insucesso do tratamento endodôntico
• Beneficiário: Pedro Ivo da Graça Fagundes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/26973-6 - Análise da capacidade de formação de biofilme e dos fatores de virulência de cepas de Enterococcus faecalis isoladas de dentes associados ao insucesso do tratamento endodôntico
• Beneficiário: João Carlos Leme Junior
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica