The use of an Allonais inaequalis reproduction test as an ecotoxicological bioassay

Autor(es)

Felipe, M. C. ^[1] ; Bernegossi, A. C. ^[1] ; Castro, G. B. ^[1] ; Pinheiro, F. R. ^[1] ; Nadai, B. L. ^[1] ; Cardoso-Silva, B. N. ^[1] ; Corbi, J. J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Hydraul & Sanitat SHS, Ecol Aquat Environm Lab, Sch Engn Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY; v. 29, n. 5, p. 634-638, JUL 2020.
• Citações Web of Science: 3

Resumo

Ecotoxicological bioassays have been widely utilized to evaluate the toxicity of substances to organisms. However, the main challenge for researchers is finding native species to assess the effects of pollutants on aquatic biota. The tropical Oligochaeta, Allonais inaequalis, can be used as a test organism in bioassays to understand the effects of toxicants on aquatic ecosystems and their impact on native aquatic biota. In this study, we tested four methodological designs to validate the use of our ``Allonais inaequalis reproduction test{''} as an ecotoxicological bioassay. For each sample, the assay consisted of a bottle containing 10 mg of sterilized fine sand, 60 mL of dechlorinated tap water and 6 organisms, fed at the beginning of the test and again after 5 days. The assay was first established in a controlled environment and then used to evaluate a stressed environment containing one of the following three toxicants suggested by the OECD (2008) and Corbi et al. (2015): zinc chloride, copper sulfate, or potassium chloride. Our results showed that the best experimental design for reproduction analysis was a static, long-term bioassay, which lasted 10 days without aeration and allowed for the reproduction of multiple generations (10 +/- 5 new organisms). The observed inhibition reproduction by toxicants (EC50 ranging between 0.2 mg L-1 and 1.36 g L-1) validated the methods used in this paper. The use of a reproduction endpoint is a new contribution to the ecotoxicological toolbox, examining responses from a native organism to predict the effects of pollutants in an aquatic environment. (AU)

• Processo FAPESP: 18/21901-0 - Avaliação do impacto ambiental de vinhaça biodigerida em reator anaeróbio utilizando testes ecotoxicológicos
• Beneficiário: Mayara Caroline Felipe
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/24622-9 - Estudo da frequência do batimento das asas do mosquito Aedes aegypti utilizando sensores inteligentes: simulação de diferentes condições ambientais em laboratório
• Beneficiário: Barbara Lepretti de Nadai
• Modalidade de apoio: Bolsas no Brasil - Doutorado