Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?

Autor(es):Mostrar menos -

Bergamo, Mariel Tavares ^[1] ; Vitor, Luciana Lourenco Ribeiro ^[2] ; Dionisio, Thiago Jose ^[3] ; Marques, Nadia Carolina Teixeira ^[4] ; Oliveira, Rodrigo Cardoso ^[3] ; Ambrosio, Eloa Cristina Passucci ^[1] ; Sakai, Vivien Thiemy ^[5] ; Santos, Carlos Ferreira ^[3] ; Lourenco Neto, Natalino ^[1] ; Machado, Maria Aparecida Andrade Moreira ^[1] ; Oliveira, Thais Marchini ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Collect Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sagrado Coracao, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[4] Jose do Rosario Vellano Univ, Sch Dent, Dept Pediat Dent, Alfenas, MG - Brazil

^[5] Univ Fed Alfenas, Sch Dent, Dept Clin & Surg, BR-37130000 Alfenas, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 36, n. 8, p. 1751-1758, OCT 2021.
• Citações Web of Science: 1

Resumo

This study aimed to evaluate the effect of different photobiomodulation (PBM) radiant exposures on the viability, proliferation, and gene expression of pulp fibroblasts from human primary teeth (HPF) involved in the pulp tissue repair. HPF were irradiated with Laser InGaAlP (Twin Flex Evolution, MMOptics (R)) at 660-nm wavelength (red); single time, continuous mode, 0.04-cm(2) laser tip area, and 0.225-cm laser tip diameter, keeping the distance of 1 mm between the laser beam and the cell culture. The doses used were between 1.2 and 6.2 J/cm(2) and were evaluated at the 6 h, 12 h, and 24 h after PBM. MTT and crystal violet assays evaluated the cell viability and proliferation. RT-PCR verified VEGF and FGF-2 mRNA expression. A blinded examiner analyzed the data through two-way ANOVA followed by Tukey test (p < 0.05). The groups with higher powers (10 mW, 15 mW, 20 mW, and 25 mW), shortest application periods (10 s), and radiant exposures between 2.5 and 6.2 J/cm(2) exhibited statistically higher viability than that of the groups with small power (5 mW), longer application period (50 s), and radiant exposure of 6.2 J/cm(2) (p < 0.05). VEGF and FGF-2 mRNA expression were observed at the three evaluated periods (6 h, 12 h, and 24 h) and the highest expression was in the shortest period (p < 0.05). All radiant exposures maintained HPF viable. The period of 6 h after irradiation showed statistically greater gene expression for both growth factors than other periods. VEGF mRNA had no differences among the dosimetries studied. The best radiant exposures for FGF-2 gene expression were 2.5 J/cm(2) and 3.7 J/cm(2). (AU)

• Processo FAPESP: 17/11396-3 - Expressão gênica de proteínas por células-tronco derivadas da polpa de dentes decíduos após o uso do laser de baixa intensidade
• Beneficiário: Mariel Tavares de Oliveira Prado Bergamo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/20316-6 - Análise da expressão gênica e síntese proteica de células derivadas da polpa de dentes decíduos após o uso do Laser de Baixa Intensidade.
• Beneficiário: Thais Marchini de Oliveira Valarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular