In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications

Autor(es)

Wachesk, C. C. ^{[1, 2]} ; Seabra, S. H. ^[3] ; Dos Santos, T. A. T. ^{[3, 4, 5]} ; Trava-Airoldi, V. J. ^[2] ; Lobo, A. O. ^[6] ; Marciano, F. R. ^[7]

Afiliação do(s) autor(es)

^[1] UNIFESP Fed Univ Sao Paulo, Dept Sci & Technol, Lab Nanotechnol & Toxicol, Sao Paulo, SP - Brazil

^[2] INPE Natl Inst Space Res, Associated Lab Sensors & Mat, Sao Paulo, SP - Brazil

^[3] UEZO Univ Estadual Zona Oeste, Technol Lab Biochem & Microscopy, Rio De Janeiro, RJ - Brazil

^[4] UENF State Univ Northern Rio de Janeiro, Lab Cell Biol & Tissue, Rio De Janeiro, RJ - Brazil

^[5] Ctr Univ IBMR, Rio De Janeiro, RJ - Brazil

^[6] UFPI Fed Univ Piaui, LIMAV Interdisciplinary Lab Adv Mat, Mat Sci & Engn Grad Program, BR-64049550 Teresina, PI - Brazil

^[7] UFPI Fed Univ Piaui, Dept Phys, BR-64049550 Teresina, PI - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 9 SEP 2021.
• Citações Web of Science: 0

Resumo

Hybrid diamond-like carbon (DLC) with incorporated titanium dioxide (TiO2) nanoparticle coatings have low friction coefficient, high wear resistance, high hardness, biocompatibility, and high chemical stability. They could be employed to modify biomedical alloys surfaces for numerous applications in biomedical engineering. Here we investigate for the first time the in vivo inflammatory process of DLC coatings with incorporated TiO2 nanoparticles. TiO2-DLC films were grown on AISI 316 stainless-steel substrates using plasma-enhanced chemical vapor deposition. The coated substrates were implanted in CF1 mice peritoneum. The in vivo cytotoxicity and biocompatibility of the samples were analyzed from macrophage lavage. Analysis in the first weeks after implantation could be helpful to evaluate the acute cytotoxicity generated after a possible inflammatory process. The in vivo results showed no inflammatory process. A significant increase in nitric oxide production on the uncoated substrates was confirmed through cytometry, and the coated substrates demonstrated biocompatibility. The presence of TiO2 nanoparticles enhanced the wound healing activity, due to their astringent and antimicrobial properties. DLC and TiO2-DLC coatings were considered biocompatible, and the presence of TiO2 nanoparticles reduced the inflammatory reactions, increasing DLC biocompatibility. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/15857-1 - Estudos científicos e aplicações inovadoras em diamante-CVD, Diamond-Like Carbon (DLC) e carbono nanoestruturado, obtidos por deposição química na fase vapor
• Beneficiário: Vladimir Jesus Trava-Airoldi
• Modalidade de apoio: Auxílio à Pesquisa - Temático