In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications

Author(s)

Wachesk, C. C. ^{[1, 2]} ; Seabra, S. H. ^[3] ; Dos Santos, T. A. T. ^{[3, 4, 5]} ; Trava-Airoldi, V. J. ^[2] ; Lobo, A. O. ^[6] ; Marciano, F. R. ^[7]

Affiliation

^[1] UNIFESP Fed Univ Sao Paulo, Dept Sci & Technol, Lab Nanotechnol & Toxicol, Sao Paulo, SP - Brazil

^[2] INPE Natl Inst Space Res, Associated Lab Sensors & Mat, Sao Paulo, SP - Brazil

^[3] UEZO Univ Estadual Zona Oeste, Technol Lab Biochem & Microscopy, Rio De Janeiro, RJ - Brazil

^[4] UENF State Univ Northern Rio de Janeiro, Lab Cell Biol & Tissue, Rio De Janeiro, RJ - Brazil

^[5] Ctr Univ IBMR, Rio De Janeiro, RJ - Brazil

^[6] UFPI Fed Univ Piaui, LIMAV Interdisciplinary Lab Adv Mat, Mat Sci & Engn Grad Program, BR-64049550 Teresina, PI - Brazil

^[7] UFPI Fed Univ Piaui, Dept Phys, BR-64049550 Teresina, PI - Brazil

• Document type: Journal article
• Source: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 9 SEP 2021.
• Web of Science Citations: 0

Abstract

Hybrid diamond-like carbon (DLC) with incorporated titanium dioxide (TiO2) nanoparticle coatings have low friction coefficient, high wear resistance, high hardness, biocompatibility, and high chemical stability. They could be employed to modify biomedical alloys surfaces for numerous applications in biomedical engineering. Here we investigate for the first time the in vivo inflammatory process of DLC coatings with incorporated TiO2 nanoparticles. TiO2-DLC films were grown on AISI 316 stainless-steel substrates using plasma-enhanced chemical vapor deposition. The coated substrates were implanted in CF1 mice peritoneum. The in vivo cytotoxicity and biocompatibility of the samples were analyzed from macrophage lavage. Analysis in the first weeks after implantation could be helpful to evaluate the acute cytotoxicity generated after a possible inflammatory process. The in vivo results showed no inflammatory process. A significant increase in nitric oxide production on the uncoated substrates was confirmed through cytometry, and the coated substrates demonstrated biocompatibility. The presence of TiO2 nanoparticles enhanced the wound healing activity, due to their astringent and antimicrobial properties. DLC and TiO2-DLC coatings were considered biocompatible, and the presence of TiO2 nanoparticles reduced the inflammatory reactions, increasing DLC biocompatibility. (AU)

• FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
• Grantee: Fernanda Roberta Marciano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 12/15857-1 - Scientific studies and innovation application on CVD diamond, DLC and carbon nanostructures obtained by chemical vapor deposition technique
• Grantee: Vladimir Jesus Trava-Airoldi
• Support Opportunities: Research Projects - Thematic Grants