Effects of curcumin-mediated antimicrobial photodynamic therapy associated to different chelators against Enterococcus faecalis biofilms

Autor(es)

Cusicanqui Mendez, Daniela Alejandra ^[1] ; Cardenas Cuellar, Maricel Rosario ^[2] ; Pedrinha, Victor Feliz ^[3] ; Velasquez Espedilla, Evelyn Giuliana ^[2] ; de Andrade, Flaviana Bombarda ^[2] ; Rodrigues, Patricia de Almeida ^[3] ; Cruvinel, Thiago ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil

^[3] Fed Univ Para, Sch Dent, Lab Dent Mat, Grad Program Dent, Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
• Citações Web of Science: 0

Resumo

Background: The aim of this study was to evaluate curcumin-mediated antimicrobial photodynamic therapy (aPDT) action combined or not with ethylenediaminetetraacetic acid (EDTA) and hydroxyethylidene bisphosphonate (HEBP) on Enterococcus faecalis biofilms. Methods: Enterococcus faecalis biofilms were grown on dentin bovine discs in brain heart infusion (BHI) medium with 1% glucose, in aerobic conditions at 37 degrees C for 7 days. Then, they were randomly distributed to one of experimental conditions, as follows: control, 75 J.cm- 2 LED, 600 Irmol.L- 1 curcumin, 17% EDTA, 18% HEBP, 600 Irmol.L-1 curcumin plus 75 J.cm- 2 LED, 600 Irmol.L- 1 curcumin plus 17% EDTA, 600 Irmol.L-1 curcumin plus 18% HEBP, 600 Irmol.L-1 curcumin plus 17% EDTA and 75 J.cm- 2 LED or 600 Irmol.L-1 curcumin plus 18% HEBP and 75 J.cm- 2 LED. The viability of microorganisms and the vitality of biofilms were determined by colony forming unit counts and confocal scanning laser microscopy (CSLM), respectively. Statistical analysis was conducted by Kruskal Wallis and Dunn's post-hoc tests (alpha = 0.05). Results: The results showed that all combinations of aPDT with chelators significantly reduced the viability of microbial cells and the vitality of biofilms in comparison to control, even when considering deeper layers of biofilms. Conclusion: The combination of curcumin with EDTA and HEBP similarly improved the effect of aPDT on E. faecalis biofilms. (AU)

• Processo FAPESP: 14/10897-0 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Thiago Cruvinel da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22238-1 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Eliézer Gutierres
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/08595-9 - Efeito da terapia fotodinâmica antimicrobiana sobre microrganismos específicos provenientes de microcosmos de lesões de cárie em dentina
• Beneficiário: Daniela Alejandra Cusicanqui Méndez
• Modalidade de apoio: Bolsas no Brasil - Mestrado