Calotropis procera (Aiton) Dryand (Apocynaceae) as an anti-cancer agent against canine mammary tumor and osteosarcoma cells

Autor(es)

Rabelo, Ana CarolinaSilveira ^[1] ; Borghesi, Jessica ^[1] ; Carreira, Ana Claudia O. ^{[1, 2]} ; Hayashi, Rafael Goncalves ^[1] ; Bessa, Fernanda ^[1] ; Nunes Barreto, Rodrigo da Silva ^[1] ; da Costa, Romario Pereira ^[3] ; Cantanhede Filho, Antonio Jose ^[3] ; Costa Carneiro, Fernando Jose ^[4] ; Miglino, Maria Angelica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Lab Stem Cell, BR-05508270 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ctr Cellular & Mol Therapy NUCEL, Sch Med, BR-05360130 Sao Paulo - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Chem, BR-13560970 Sao Carlos - Brazil

^[4] Fed Inst Educ Sci & Technol Maranhao, Dept Chem, Campus Sao Luis, BR-65030005 Monte Castelo, Maranhao - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 138, p. 79-89, SEP 2021.
• Citações Web of Science: 0

Resumo

Our goal was to evaluate phytochemical characterization and the antitumor potential of Calotropis procera. The phytochemical constitution of the crude extract (CE) revealed the presence of flavonoids, glycosides and cardenolide. The MTT assay was used to evaluate the cytotoxicity of CE, methanolic (MF) and ethyl acetate fractions (EAF) of C. procera in canine osteosarcoma cells (OST), canine mammary tumor (CMT), and canine skin fibroblasts (non-tumor cell). Doxorubicin was also used as a positive control. Results showed that CE, MF and EAF promoted a decrease in the viability of OST and CMT cells and did not alter the fibroblasts viability. C. procera also decreased the number of cells, corroborating to the decrease in proliferation and the cell cycle arrest in the G0/G1 phase. It was also evaluated the cell morphology by light and fluorescence microscopy, being demonstrated a reduction in cytoplasmic and cell rounding characteristic of programmed cell death. Moreover, flow cytometry data demonstrated that CE treatment promoted increase of caspase-3 and p53, showing that the cell death was activated in OST cells. In addition, there was a decrease in CD31, VEGF, osteopontin and TGF-beta after CE treatment, suggesting that CE exerts its antitumor effect by reducing angiogenesis and tumor progression in OST cells. Moreover, CMT cells showed a reduction in PCNA after treatment with MF and CE. Analyzing the data together, C. procera, especially CE, showed an antitumor potential in both OST and CMT cells, encouraging us to continue investigating its use in cancer therapy. (AU)

• Processo FAPESP: 14/50844-3 - Matriz extracelular na saúde e matriz placentária na regeneração de tecidos
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Temático