Clinically translatable quantitative molecular photoacoustic imaging with liposome-encapsulated ICG J-aggregates

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Wood, Cayla A. ^{[1, 2]} ; Han, Sangheon ^{[1, 3]} ; Kim, Chang Soo ^[1] ; Wen, Yunfei ^[4] ; Sampaio, Diego R. T. ^{[1, 5]} ; Harris, Justin T. ^[6] ; Homan, Kimberly A. ^[6] ; Swain, Jody L. ^[7] ; Emelianov, Stanislav Y. ^{[8, 9, 10]} ; Sood, Anil K. ^{[2, 4]} ; Cook, Jason R. ^[6] ; Sokolov, V, Konstantin ; Bouchard, Richard R. ^{[11, 12]}

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^[1] Univ Texas MD Anderson Canc Ctr, Dept Imaging Phys, Houston, TX 77030 - USA

^[2] Univ Texas MD Anderson Canc Ctr UTHlth, Grad Sch Biomed Sci, Houston, TX 77030 - USA

^[3] V, Rice Univ, Dept Bioengn, Houston, TX 77005 - USA

^[4] Univ Texas MD Anderson Canc Ctr, Dept Gynecol Oncol & Reprod Med, Houston, TX 77030 - USA

^[5] Univ Sao Paulo, Dept Phys, Ribeirao Preto - Brazil

^[6] NanoHybrids Inc, Austin, TX - USA

^[7] Univ Texas MD Anderson Canc Ctr, Dept Vet Med & Surg, Houston, TX 77030 - USA

^[8] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 - USA

^[9] Emory Univ, Sch Med, Atlanta, GA - USA

^[10] Georgia Inst Technol, Sch Elect & Comp Engn, Atlanta, GA 30332 - USA

^[11] Sokolov, Konstantin, V, Univ Texas MD Anderson Canc Ctr, Dept Imaging Phys, Houston, TX 77030 - USA

^[12] Sokolov, Konstantin, V, Univ Texas MD Anderson Canc Ctr UTHlth, Grad Sch Biomed Sci, Houston, TX 77030 - USA

• Tipo de documento: Artigo Científico
• Fonte: NATURE COMMUNICATIONS; v. 12, n. 1 SEP 13 2021.
• Citações Web of Science: 0

Resumo

Photoacoustic imaging is limited by a lack of contrast agents which can enable combined molecular and physiological imaging at depth. Here the authors address these limitations by developing and validating a contrast agent based on targeted liposomes loaded with J-aggregated indocyanine green dye. Photoacoustic (PA) imaging is a functional and molecular imaging technique capable of high sensitivity and spatiotemporal resolution at depth. Widespread use of PA imaging, however, is limited by currently available contrast agents, which either lack PA-signal-generation ability for deep imaging or their absorbance spectra overlap with hemoglobin, reducing sensitivity. Here we report on a PA contrast agent based on targeted liposomes loaded with J-aggregated indocyanine green (ICG) dye (i.e., PAtrace) that we synthesized, bioconjugated, and characterized to addresses these limitations. We then validated PAtrace in phantom, in vitro, and in vivo PA imaging environments for both spectral unmixing accuracy and targeting efficacy in a folate receptor alpha-positive ovarian cancer model. These study results show that PAtrace concurrently provides significantly improved contrast-agent quantification/sensitivity and SO2 estimation accuracy compared to monomeric ICG. PAtrace's performance attributes and composition of FDA-approved components make it a promising agent for future clinical molecular PA imaging. (AU)

• Processo FAPESP: 16/22720-3 - Ondas fotoacústicas e radiação ionizante: imagens fotoacústicas por laser e raios-X
• Beneficiário: Diego Ronaldo Thomaz Sampaio
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado