Cubosomal lipid nanoassemblies with pH-sensitive shells created by biopolymer complexes: A synchrotron SAXS study

Autor(es)

Mathews, Patrick D. ^[1] ; Mertins, Omar ^{[1, 2]} ; Angelov, Borislav ^[3] ; Angelova, Angelina ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Paulista Med Sch, Dept Biophys, Lab Nano Bio Mat LNBM, BR-04023062 Sao Paulo - Brazil

^[2] Univ Paris Saclay, Inst Galien Paris Saclay, CNRS, UMR8612, F-92296 Chatenay Malabry - France

^[3] Acad Sci Czech Republ, Inst Phys, ELI Beamlines, CZ-18221 Prague - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: Journal of Colloid and Interface Science; v. 607, n. 1, p. 440-451, FEB 2022.
• Citações Web of Science: 0

Resumo

We report a strategy for sustainable development of pH-responsive cubic liquid crystalline nanoparticles (cubosomes), in which the structure-defining lyotropic nonlamellar lipid and the eventually encapsulated guest molecules can be protected by pH-sensitive polyelectrolyte shells with mucoadhesive properties. Bulk non-lamellar phases as well as pH-responsive polyelectrolyte-modified nanocarriers were formed by spontaneous assembly of the nonlamellar lipid monoolein and two biopolymers tailored in nanocom-plexes with pH-dependent net charge. The mesophase particles involved positively charged N-arginine-modified chitosan (CHarg) and negatively charged alginate (ALG) chains assembled at different biopoly-mer concentrations and charge ratios into a series of pH-responsive complexes. The roles of Pluronic F127 as a dispersing agent and a stabilizer of the nanoscale dispersions were examined. Synchrotron small-angle X-ray scattering (SAXS) investigations were performed at several N-arginine-modified chitosan/al-ginate ratios (CHarg/ALG with 10,15 and 20 wt% ALG relative to CHarg) and varying pH values mimicking the pH conditions of the gastrointestinal route. The structural parameters characterizing the inner cubic liquid crystalline organizations of the nanocarriers were determined as well as the particle sizes and sta-bility on storage. The surface charge variations, influencing the measured zeta-potentials, evidenced the inclusion of the CHarg/ALG biopolymer complexes into the lipid nanoassemblies. The polyelectrolyte shells rendered the hybrid cubosome nanocarriers pH-sensitive and influenced the swelling of their lipid phase core as revealed by the acquired SAXS patterns. The pH-responsiveness and the mucoadhesive features of the cubosomal lipid/polyelectrolyte nanocomplexes may be of interest for in vivo drug delivery applications. (c) 2021 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 19/13147-6 - Estudos dos genes imunes expressos diferencialmente em órgãos de peixes ornamentais em resposta à infecção por mixosporídeos
• Beneficiário: Patrick Mathews Delgado
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 21/00971-2 - Nano cubossoma de fase cristalina e funcionalizado com biopolímeros: desenvolvimento como carreador de fármaco e estudos in vivo em zebrafish (Danio rerio)
• Beneficiário: Omar Mertins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/21727-0 - Estudo e desenvolvimento de cubossomas com polímeros biofuncionais pH-responsivos como carreadores de fármacos e agentes bioativos
• Beneficiário: Omar Mertins
• Modalidade de apoio: Bolsas no Exterior - Pesquisa