Nandrolone combined with strenuous resistance training impairs myocardial proteome profile of rats

Autor(es)

Dantas, Patricia Sousa ^[1] ; Guzzoni, Vinicius ^[2] ; Perez, Juliana Dineia ^[1] ; Arita, Danielle Yuri ^[1] ; Novaes, Pedro Duarte ^[3] ; Marcondes, Fernanda Klein ^[4] ; Casarini, Dulce Elena ^[1] ; Cunha, Tatiana Sousa ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Med, Nephrol Div, Sao Paulo, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Dept Sci & Technol, Sao Jose Dos Campos, SP - Brazil

^[3] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Morphol, Piracicaba, SP - Brazil

^[4] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Steroids; v. 175, NOV 2021.
• Citações Web of Science: 0

Resumo

We aimed to investigate the effects of high doses of nandrolone decanoate and resistance training (RT) on the proteomic profile of the left ventricle (LV) of rats, using a label-free quantitative approach. Male rats were randomized into four groups: untrained vehicle (UTV), trained vehicle (TV), untrained nandrolone (UTN), and trained nandrolone (TN). Rats were familiarized with the exercise training protocol (jump exercise) for one week. Jump-exercise was performed five days a week for 6 weeks, with 30 s of inter-set rest intervals. Nandrolone was administrated for 6 weeks (5 mg/kg, twice a week, via intramuscular). Systolic and diastolic arterial pressure and heart rate were measured 48 h post-training. LV was isolated and collagen content was measured. The expression of cardiac proteins was analyzed by ultra-efficiency liquid chromatography with mass spectrometry high / low collision energy (UPLC/MSE). Nandrolone and RT led to cardiac hypertrophy, even though high doses of nandrolone counteracted the RT-induced arterial pressures lowering. Nandrolone also affected the proteome profile negatively in LV of rats, including critical proteins related to biological processes (metabolism, oxidative stress, inflammation), structural function and membrane transporters. Our findings show physiological relevance since high doses of nandrolone induced detrimental effects on the proteome profile of heart tissue and hemodynamic parameters of rats. Furthermore, as nandrolone abuse has become increasingly common among recreational athletes and casual fitness enthusiasts, we consider that our findings have clinical relevance as well. (AU)

• Processo FAPESP: 05/60284-6 - Respostas cardiovasculares ao esteroide anabolico nandrolona, associado a treinamento fisico anaerobio.
• Beneficiário: Fernanda Klein Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular