Three-dimensional-printed millireactor with yeast immobilized in calcium-alginate film for application in fermentation processes

Autor(es)

Lopes, Mariana G. M. ^[1] ; Santana, Harrson S. ^[1] ; da Silva, Adriano G. P. ^[1] ; Taranto, Osvaldir P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AICHE JOURNAL; v. 68, n. 1 OCT 2021.
• Citações Web of Science: 0

Resumo

An innovative method of alcoholic fermentation was proposed by developing a microfluidic device with millichannels filled with yeast immobilized in alginate hydrogel, manufactured by a new robotic system. We propose and build an automatic equipment consisting of a modified three-dimensional (3D) printer with two independent systems, a module with a syringe for the injection of solutions/hydrogels and a conventional head with a fused filament fabrication mechanism. The methodology consisted of hydrogel 3D printing tests, the manufacture of millireactors with immobilized yeast and the application of the millireactors in alcoholic fermentation. The 22G needle provided the best printing accuracy in tests with hydrogel. The cells were homogeneously distributed by alginate and their concentration did not influence ethanol production. The optimal millireactor configuration was 2% alginate solution containing 25 g L-1 cells, achieved average ethanol production of 11.24 +/- 0.015 g L-1, with productivity of 22.49 g L-1 h(-1) and efficiency of 44%. (AU)

• Processo FAPESP: 16/20842-4 - Desenvolvimento de uma microplanta química para produção de biodiesel baseada em microrreatores
• Beneficiário: Osvaldir Pereira Taranto
• Modalidade de apoio: Auxílio à Pesquisa - Regular