Autor(es)

Harada, Liliam K. ^[1] ; Junior, Waldemar Bonventi ^[2] ; Silva, Erica C. ^[1] ; Oliveira, Thais J. ^[1] ; Moreli, Fernanda C. ^[1] ; Junior, Jose M. Oliveira ^[1] ; Tubino, Matthieu ^[3] ; Vila, Marta M. D. C. ^[1] ; Balcao, Victor M. ^{[4, 5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, Lab Biofilms & Bacteriophages, PhageLab, BR-18023000 Sorocaba, SP - Brazil

^[2] Fac Technol Sorocaba FATEC SO, BR-18013280 Sorocaba, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[4] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro - Portugal

^[5] Univ Aveiro, CESAM, Campus Univ Santiago, P-3810193 Aveiro - Portugal

• Tipo de documento: Artigo Científico
• Fonte: BIOSENSORS-BASEL; v. 11, n. 4 APR 2021.
• Citações Web of Science: 1

Resumo

During the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet's biosphere, killing between 4-50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacterial infections linked to healthcare services are widespread, which, when associated with the increasing surge of antibiotic-resistant microorganisms, play a major role in patient morbidity and mortality. In this scenario, Pseudomonas aeruginosa alone is responsible for ca. 13-15% of all hospital-acquired infections. The pathogen P. aeruginosa is an opportunistic one, being endowed with metabolic versatility and high (both intrinsic and acquired) resistance to antibiotics. Bacteriophages (or phages) have been recognized as a tool with high potential for the detection of bacterial infections since these metabolically inert entities specifically attach to, and lyse, bacterial host cells, thus, allowing confirmation of the presence of viable cells. In the research effort described herein, three different phages with broad lytic spectrum capable of infecting P. aeruginosa were isolated from environmental sources. The isolated phages were elected on the basis of their ability to form clear and distinctive plaques, which is a hallmark characteristic of virulent phages. Next, their structural and functional stabilization was achieved via entrapment within the matrix of porous alginate, biopolymeric, and bio-reactive, chromogenic hydrogels aiming at their use as sensitive matrices producing both color changes and/or light emissions evolving from a reaction with (released) cytoplasmic moieties, as a bio-detection kit for P. aeruginosa cells. Full physicochemical and biological characterization of the isolated bacteriophages was the subject of a previous research paper. (AU)

• Processo FAPESP: 16/08884-3 - PneumoPhageColor - desenvolvimento de um kit de biodetecção colorimétrica de Pseudomonas aeruginosa com base em partículas fágicas
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/12234-4 - TransAppIL - Permeação cutânea de entidades proteicas estabilizadas estruturalmente e funcionalmente, carreadas por líquidos iônicos.
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/05522-9 - PsaPhageKill - isolamento, caracterização e uso de bacteriófagos líticos contra Pseudomonas syringae pv. actinidiae para combater o cancro do kiwi: uma alternativa eficiente e amiga do ambiente
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Bolsas no Exterior - Pesquisa