Luminescent imaging of insulin amyloid aggregation using a sensitive ruthenium-based probe in the red region

Autor(es)

Pereira, Lorena M. B. ^[1] ; Cali, Mariana P. ^[1] ; Marchi, Rafael C. ^[1] ; Pazin, Wallance M. ^[2] ; Carlos, Rose M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Paulista, Dept Phys, BR-19060900 Presidente Prudente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Inorganic Biochemistry; v. 224, NOV 2021.
• Citações Web of Science: 0

Resumo

A sensitive and selective strategy to identify insulin fibrils remains a challenge for researchers in amyloid protein research. Thus, it is critical to detect, in vitro, the species generated during amyloid aggregation, particularly the fibrillar species. Here we demonstrate that the luminescent complex cis-{[}Ru(phen)(2)(3,4Apy)(2)](2+) (RuApy; phen = 1,10-phenanthroline; 3,4Apy = 3,4-diaminopyridine) is a rapid, low-cost alternative to in vitro detection of fibrillar insulin, using conventional optical techniques. The RuApy complex displays emission intensity enhancement at 655 nm when associated with insulin, which enables imaging of the conformational changes of the protein's self-aggregation. The complex shows high sensitivity to fibrillar insulin with a limit of detection of 0.85 mu M and binding affinity of 12.40 +/- 1.84 mu M which is comparable to those of Thioflavin T and Congo red, with the advantage of minimizing background fluorescence, absorption of light by biomolecules, and light scattering from physiologic salts in the medium. (AU)

• Processo FAPESP: 20/12129-1 - Imunoensaio para diagnóstico da COVID-19 usando espectroscopia de espalhamento Raman amplificado em superfície (SERS)
• Beneficiário: Wallance Moreira Pazin
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/21143-0 - Agregados de proteínas amiloides e a relação entre a Doença de Alzheimer e Diabetes tipo 2 investigada por complexos luminescentes de Ru(II)
• Beneficiário: Rose Maria Carlos
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/00839-1 - Análise da relação estrutura-toxicidade de peptídeos b-amiloide e efeito neuroprotetivo de complexos de Ru(II) luminescentes
• Beneficiário: Rose Maria Carlos
• Linha de fomento: Auxílio à Pesquisa - Regular