| Texto completo | |
| Autor(es): |
Xavier Guilherme, Ederson Paulo
[1]
;
Zanphorlin, Leticia Maria
[2]
;
Sousa, Amanda Silva
[2]
;
Miyamoto, Renan Yuji
[3, 2]
;
Oliveira Bruziquesi, Carlos Giovani
[4]
;
Aparecida de Carvalho Mesquita, Bruna Mara
[1]
;
Sousa Santos, Sergio Henrique
[1]
;
Aguiar-Oliveira, Elizama
[5]
;
Cota, Junio
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Fed Minas Gerais, Inst Agr Sci, Montes Claros, MG - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil
[4] Univ Fed Minas Gerais, Dept Chem, Belo Horizonte, MG - Brazil
[5] Univ Estadual Santa Cruz, Dept Exact Sci & Technol, Ilheus, BA - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | RENEWABLE ENERGY; v. 182, p. 274-284, JAN 2022. |
| Citações Web of Science: | 0 |
| Resumo | |
Integrated bioprocess strategies may facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. We propose a new process concept, SSICF, where sugarcane bagasse is hy-drolyzed simultaneously with xylose isomerization and the co-fermentation of C6 and C5 sugars. A commercial cocktail was supplemented with our multi-enzymatic system composed of three recombi-nant enzymes immobilized in Feroxyhyte magnetic nanoparticles: b-glucosidase, b-xylosidase and xylose isomerase. SSICF was performed using non-GMO Saccharomyces at pH 6.0 and 35 degrees C for 72 h in a synthetic medium containing cellobiose and xylose, and another medium containing pretreated sugar-cane bagasse (PSB). The results of ethanol global yields in SSICF were 77.67% and 73.24% for the synthetic medium and PSB, respectively. In a nutshell, this is the first report of a successful proof-of-concept of SSICF with four rounds of enzyme recycling and a non-GMO yeast, an innovative process with high potential for industrial use. (C) 2021 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 18/02865-2 - Investigação molecular de novas xilose isomerases para aplicação na fermentação de materiais lignocelulósicos |
| Beneficiário: | Renan Yuji Miyamoto |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 19/08855-1 - Novos mecanismos de P450: uma estratégia enzimática para a produção de hidrocarbonetos renováveis |
| Beneficiário: | Leticia Maria Zanphorlin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |