requency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinom

Autor(es):Mostrar menos -

Munari, Fernanda Franco ^[1] ; Sichero, Laura ^[2] ; Carloni, Adriana Cruvinel ^[1] ; Lacerda, Croider Franco ^{[1, 3]} ; Nunes, Emily Montosa ^[2] ; Torres de Oliveira, Antonio Talvane ^[3] ; Scapulatempo-Neto, Cristovam ^{[1, 4]} ; Morini da Silva, Sandra Regina ^[4] ; Crema, Eduardo ^[5] ; Adad, Sheila Jorge ^[6] ; Marchesan Rodrigues, Maria Aparecida ^[6] ; Coelho Arruda Henry, Maria Aparecida ^[6] ; Guimaraes, Denise Peixoto ^{[1, 7]} ; Reis, Rui Manuel ^{[8, 1, 9]} ; Villa, Luisa Lina ^{[10, 2]} ; Longatto-Filho, Adhemar ^{[1, 9, 11, 12]}

Afiliação do(s) autor(es):Mostrar menos -

^[1] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos - Brazil

^[2] Inst Canc Estado Sao Paulo ICESP, Ctr Translat Res Oncol, Sao Paulo - Brazil

^[3] Barretos Canc Hosp, Dept Digest Surg, Barretos - Brazil

^[4] Barretos Canc Hosp, Dept Pathol Diag Biopsies & Surg Specimens, Barretos - Brazil

^[5] Univ Fed Triangulo Mineiro, Dept Digest Surg & Pathol, Med Sch, UFTM, Uberaba - Brazil

^[6] Sao Paulo State Univ, Med Sch, Dept Gastroenterol Surg & Pathol, UNESP, Botucatu, SP - Brazil

^[7] Barretos Canc Hosp, Dept Endoscopy, Barretos - Brazil

^[8] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

^[9] Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, Braga - Portugal

^[10] Univ Sao Paulo, Fac Med, Dept Radiol & Oncol, Sao Paulo - Brazil

^[11] Univ Sao Paulo, Med Sch, Dept Radiol & Oncol, Butanta - Brazil

^[12] Univ Sao Paulo, Med Lab Med Invest LIM 14, Dept Pathol, Med Sch, Butanta - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOBIOLOGY; OCT 2021.
• Citações Web of Science: 0

Resumo

Background: Chagasic megaesophagus (CM) as well as the presence of human papillomavirus (HPV) has been reported as etiological factors for esophageal squamous cell carcinoma (ESCC). Objective: We assessed the prevalence of HPV DNA in a series of ESCCs associated or not with CM. Data obtained were further correlated to the pathological and clinical data of affected individuals. Methods: A retrospective study was performed on 92 formalin-fixed and paraffin-embedded tissues collected from patients referred to 3 different hospitals in Sao Paulo, Brazil: Barretos Cancer Hospital, Barretos, Sao Paulo; Federal University of Triangulo Mineiro, Uberaba, Minas Gerais; and Sao Paulo State University, Botucatu, Sao Paulo. Cases were divided into 3 groups: (i) 24 patients with CM associated with ESCC (CM/ESCC); (ii) 37 patients with ESCC without CM (ESCC); and (iii) 31 patients with CM without ESCC (CM). Detection of HPV DNA was assessed in all samples by a genotyping assay combining multiplex polymerase chain reaction and bead-based Luminex technology. Results: We identified a high prevalence of high-risk HPV in patients in the CM group (12/31, 38.8%) and CM/ESCC (8/24, 33.3%), compared to individuals in the ESCC group (6/37, 16.3%). The individuals in the groups with cancer (ESCC and CM/ESCC) had a higher frequency of HPV-16 (4/9, 44.5% and 2/8, 25.0%). The other types of high-risk HPVs detected were HPV-31, 45, 51, 53, 56, 66, and 73. We also observed in some samples HPV coinfection by more than one viral type. Despite the high incidence of HPV, it did not show any association with the patient's clinical-pathological and molecular (TP53 mutation status) characteristics. Conclusion: This is the first report of the presence of HPV DNA in CM associated with ESCC. HPV infection was more presence in megaesophagus lesions. Further studies are needed to confirm and better understand the role of persistent HPV infection in patients with CM. </p> (AU)

• Processo FAPESP: 15/20077-3 - Detecção de DNA de HPV de alto risco e análises do status mutacional dos genes KRAS e PIK3CA e do painel de citocinas inflamatórias em pacientes com câncer de esôfago e megaesôfago chagásico com e sem câncer
• Beneficiário: Fernanda Franco Munari
• Modalidade de apoio: Bolsas no Brasil - Mestrado