Zinc pthalocyanine loaded poly (lactic acid) nanoparticles by double emulsion methodology for photodynamic therapy against 9 L/LacZ gliosarcoma cells

Autor(es)

de Oliveira, Benedito Marcio ^[1] ; Miranda Teodoro, Jessica Beatriz ^[1] ; Ribeiro Ambrosio, Jessica Aparecida ^[1] ; Goncalves, Erika Peterson ^[1] ; Beltrame, Milton ^[1] ; Cortez Marcolino, Luciana Maria ^[2] ; Pinto, Juliana Guerra ^[2] ; Ferreira-Strixino, Juliana ^[2] ; Simioni, Andreza Ribeiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Res & Dev, Organ Synth Lab, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos - Brazil

^[2] Univ Vale Paraiba, Inst Res & Dev, Lab Photobiol Appl Hlth, Sao Jose Dos Campos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION; v. 33, n. 1 SEP 2021.
• Citações Web of Science: 1

Resumo

Development delivery systems, such as nanoparticles, represent a growing area in biomedical research. Nanoparticles (NP) were prepared using a double-emulsion method to load zinc(II) phthalocyanine (ZnPc). NP were obtained using poly (lactic acid) (PLA). ZnPc is a second generation of photosensitizer used in photodynamic therapy (PDT). ZnPc loaded PLA nanoparticles (NPLA-ZnPc) were prepared by double-emulsion method, characterized and available in cellular culture. The mean nanoparticle size presented particle size was 384.7 +/- 84.2 nm with polydispersity index (PDI) of 0.150 +/- 0.015, and the encapsulation efficiency was of 83%. The nanoparticle formulations presented negative zeta potential values (-27.5 +/- 1.0 mV), explaining their colloidal stability. ZnPc loaded nanoparticles maintain its photophysical behavior after encapsulation. Photosensitizer release from nanoparticles was sustained over 168 h with a biphasic ZnPc release profile. An in vitro phototoxic effect in range of 80% was observed in 9 L/LacZ gliosarcoma cells at laser light doses (10 J cm(-2)) with 3.0 mu g mL(-1) of NPLA-ZnPc. All the physical-chemical, photophysical and photobiological measurements performed allow us to conclude that ZnPc loaded PLGA nanoparticles is a promising drug delivery system for PDT. (AU)

• Processo FAPESP: 18/18531-6 - Microcápsulas polieletrolíticas como modelo de sistema de liberação controlada para fármacos fotossensíveis
• Beneficiário: Andreza Ribeiro Simioni
• Modalidade de apoio: Auxílio à Pesquisa - Regular