Profile of host cell responses to exposure to stressed bacteria in planktonic; dislodged, and intact biofilm mode

Autor(es)

Rafaela Fernandes Zancan ^[1] ; José Burgos Ponce ^[2] ; Thiago José Dionisio ^[3] ; Rodrigo Cardoso de Oliveira ^[4] ; Rafaela Alves da Silva ^[5] ; Jussaro Alves Duque ^[6] ; Marco Antonio Hungaro Duarte ^[7]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

^[2] University of São Paulo. Bauru Dental School. Department of Surgery, Stomatology, Pathology and Radiology - Brasil

^[3] University of São Paulo. Bauru Dental School. Department of Biological Sciences - Brasil

^[4] University of São Paulo. Bauru Dental School. Department of Pediatric Dentistry, Orthodontics and Public Health - Brasil

^[5] University of São Paulo. Bauru Dental School. Department of Pediatric Dentistry, Orthodontics and Public Health - Brasil

^[6] University of São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

^[7] University of São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 32, n. 3, p. 10-20, 2021-11-08.

Resumo

Abstract The host defense response to microbial challenge emerging from the root canal system leads to apical periodontitis. The aim of this study was to evaluate the expression of inflammatory cytokines and Nitric Oxide (NO) by macrophages after interaction with Enterococcus faecalis in the: plankton and dislodged biofilm mode; intact biofilm mode stimulated by calcium hydroxide (CH), CH and chlorhexidine (CHX) or Triple Antibiotic Paste (TAP). For this purpose, culture of macrophages from monocytes in human peripheral blood (N=8) were exposed to the different modes of bacteria for 24 hours. Subsequently, the cytokines, such as, Tumor Necrotic Factor- alfa (TNF-α), interleukin (IL)-1β, IL-6, IL-10; and NO were quantified by Luminex xMAP and Greiss reaction, respectively. In addition to the potential therapeutic effects of the intracanal medication, their antimicrobial activity against Enterococcus faecalis biofilm were also tested in vitro by confocal microscopy. The experiments` data were analyzed by the Kruskal-Wallis test with the Dunn post hoc test (α < 0.05). Bacteria in dislodged biofilm mode were shown to be more aggressive to the immune system than bacteria in plankton mode and negative control, inducing greater expression of NO and TNF-α. Relative to bacteria in intact biofilm mode, the weakest antimicrobial activity occurred in Group CH. In Groups CH/CHX and TAP the percentage of dead bacteria was significantly increased to the same extent. Interestingly, the biofilm itself did not induce the release of pro-inflammatory cytokines - except for NO - while the biofilm treated with TAP and CH based pastes enhanced the levels of IL-6 and TNF-α; and IL-1 β, respectively. In contrast, the levels of a potent anti-inflammatory (IL-10) were increased in Group TAP. (AU)

• Processo FAPESP: 16/25133-1 - Enterococcus faecalis: Desenvolvimento de irrigante final para destruí-lo, análise de sua sobrevivência após tratamento endodôntico e sua capacidade de estimular citocinas pró-inflamatórias após ser estressado.
• Beneficiário: Rafaela Fernandes Zancan
• Modalidade de apoio: Bolsas no Brasil - Doutorado