| Texto completo | |
| Autor(es): |
Zocca, Vitoria Fernanda Bertolazzi
[1]
;
Correa, Graciely Gomes
[1]
;
Lins, Milca Rachel da Costa Ribeiro
[1]
;
de Jesus, Victor Nunes
[1]
;
Tavares, Leonardo Ferro
[1]
;
Amorim, Laura Araujo da Silva
[1]
;
Kundlatsch, Guilherme Engelberto
[1]
;
Pedrolli, Danielle Biscaro
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista UNESP, Sch Pharmaceut Sci, Dept Bioproc Engn & Biotechnol, Araraquara, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo de Revisão |
| Fonte: | CRITICAL REVIEWS IN BIOTECHNOLOGY; OCT 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
CRISPR has revolutionized the way we engineer genomes. Its simplicity and modularity have enabled the development of a great number of tools to edit genomes and to control gene expression. This powerful technology was first adapted to Bacillus subtilis in 2016 and has been intensely upgraded since then. Many tools have been successfully developed to build a CRISPR toolbox for this Gram-positive model and important industrial chassis. The toolbox includes tools, such as double-strand and single-strand cutting CRISPR for point mutation, gene insertion, and gene deletion up to 38 kb. Moreover, catalytic dead Cas proteins have been used for base editing, as well as for the control of gene expression (CRISPRi and CRISPRa). Many of these tools have been used for multiplex CRISPR with the most successful one targeting up to six loci simultaneously for point mutation. However, tools for efficient multiplex CRISPR for other functionalities are still missing in the toolbox. CRISPR engineering has already resulted in efficient protein and metabolite-producing strains, demonstrating its great potential. In this review, we cover all the important additions made to the B. subtilis CRISPR toolbox since 2016, and strain developments fomented by the technology. (AU) | |
| Processo FAPESP: | 18/24733-0 - Desenvolvimento de dispositivo kill switch foto-ativado para biocontenção de microrganismos geneticamente modificados |
| Beneficiário: | Victor Nunes de Jesus |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 14/17564-7 - Reprogramação do metabolismo de purina em Bacillus subtilis através de tecnologia de sRNA |
| Beneficiário: | Danielle Biscaro Pedrolli |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 20/16058-1 - Otimização de um circuito genético responsivo à glicose |
| Beneficiário: | Leonardo Ferro Tavares |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |