New Insights into Controlling Homoacetogenesis in the Co-digestion of Coffee Waste: Effect of Operational Conditions and Characterization of Microbial Communities

Autor(es)

Villa Montoya, Alejandra Carolina ^[1] ; da Silva Mazareli, Raissa Cristina ^[1] ; Delforno, Tiago Palladino ^[2] ; Centurion, Victor Borin ^[3] ; de Oliveira, Valeria Maia ^[3] ; Silva, Edson Luiz ^[4] ; Amancio Varesche, Maria Bernadete ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Lab Biol Proc, Campus 2, BR-13563120 Sao Carlos, SP - Brazil

^[2] SENAI Innovat Inst Biotechnol, BR-01130000 Bom Retiro, SP - Brazil

^[3] Univ Estadual Campinas, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Campinas, SP - Brazil

^[4] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; NOV 2021.
• Citações Web of Science: 0

Resumo

In this research batch reactors were operated with coffee processing waste and autochthonous microbial consortium, and a taxonomic and functional analysis was performed for phase I of stabilization of maximum H-2 production and for phase II of maximum H-2 consumption. During phase I, the reactor's operating conditions were pH 4.84 to 8.18, headspace 33.18% to 66.82%, and pulp and husk from 6.95 to 17.05 g/L. These assays continued for phase II, with initial pH conditions of 5.8-8.1, headspace of 33.18-66.82%, and pulp and husk remaining from phase I. The highest homoacetogenesis was observed in assay 5 with pH 7.7, 40% headspace, and 15 g/L of pulp and husk (initial concentrations of phase I). A relative abundance of Clostridium 41%, Lactobacillus 20% and Acetobacter 14% was observed in phase I. In phase II, there was a change in relative abundance of 21%, 63%, and 1%, respectively, and functional genes involved with autotrophic (formyltetrahydrofolate synthase) and heterotrophic (enolase) homoacetogenesis, butanol (3-hydroxybutyryl-CoA dehydrogenase), and propionic acid (propionate CoA-transferase) were identified. This study provides a new and amplified insight into the physicochemical and microbiological factors, which can be used to propose adequate operational conditions to maximize the bioenergy production and reduce homoacetogenesis in biological reactors. (AU)

• Processo FAPESP: 16/20047-0 - Avaliação das caraterísticas físico-químicas e filogenéticas da produção de hidrogênio e homoacetogênese a partir de resíduos do processamento de café
• Beneficiário: Alejandra Carolina Villa Montoya
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06246-7 - Aplicação do conceito de biorrefinaria a estações de tratamento biológico de águas residuárias: o controle da poluição ambiental aliado à recuperação de matéria e energia
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático