he use of probiotics can reduce the severity of experimental periodontitis in rats with metabolic syndrome: An immunoenzymatic and microtomographic stud

Autor(es)

Silva, Giselle A. ^[1] ; Moreira, Andre L. G. ^[1] ; Silva, Pedro H. F. ^[1] ; Salvador, Sergio L. ^[2] ; Casarin, V, Renato C. ; Vicente, Raphael M. ^[3] ; Ferreira, Graziele C. ^[4] ; Tanus-Santos, Jose E. ^[4] ; Furlaneto, Flavia A. C. ^[1] ; Messora, Michel R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, USP, Av Cafe S-N, BR-14020150 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal, USP, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Orthoped & Anesthesiol, USP, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, USP, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 93, n. 2 NOV 2021.
• Citações Web of Science: 0

Resumo

Background This study evaluated the effects of Bifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) in the development of periodontitis (PE), associated or not with metabolic syndrome, (MS) in rats. Methods Ninety-six rats were grouped according to a food protocol: high-fat diet for induction of MS or standard diet for the control groups (C). They were subdivided into groups with (+) and without (-) PE, receiving ({*}) or not ({*}{*}) probiotic (PROB): C-{*}{*}, CP-{*}, PE+{*}{*}, PEP+{*}, MS- MSP-{*}, MSPE+{*}{*}, and MSPEP+{*}. PROB administration started on the eighth week of the study and PE was induced on the 14th week by placing ligature on the animals' lower first molars. Euthanasia occurred in the 16th week. Biomolecular analyzes, immunoenzymatic assays, and microtomographic analyses were performed. The data obtained were analyzed statistically (P < 0.05). Results The PEP and MSPEP groups showed lower levels of alveolar bone loss when compared with the PE and MSPE groups, respectively (P < 0.05). The immunoenzymatic analysis showed higher levels of interleukin (IL)-1 beta and a higher receptor activator of NF-kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio in the MSPE group when compared with the MSPEP group (P < 0.05). The PEP group showed lower levels of tumor necrosis factor (TNF)-alpha and IL-6 when compared with the PE group. The use of PROB attenuated dyslipidemia parameters in animals with MS, with or without PE. Conclusion B. lactis HN019 reduced more significantly the severity of PE in rats with MS, modulating both systemic metabolic and immunoinflammatory parameters in periodontal tissues. (AU)

• Processo FAPESP: 18/16009-0 - Efeitos da terapia probiótica na periodontite experimental em ratos portadores de síndrome metabólica induzida por meio de dieta hiperlipídica
• Beneficiário: Giselle Aparecida da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/26257-9 - Efeitos da terapia com Bifidobacterium animalis subsp. lactis HN019 na periodontite experimental associada à Síndrome Metabólica. Estudo do perfil microtomográfico, histomorfométrico, imunoenzimático e de expressão gênica em ratos.
• Beneficiário: Michel Reis Messora
• Modalidade de apoio: Auxílio à Pesquisa - Regular