Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

Autor(es)

Garcia, Maira Terra ^[1] ; Ward, Rafael Araujo da Costa ^[1] ; Goncalves, Nathalia Maria Ferreira ^[1] ; Pedroso, Lara Luise Castro ^[1] ; Neto, Jose Vieira da Silva ^[2] ; Strixino, Juliana Ferreira ^[3] ; Junqueira, Juliana Campos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos - Brazil

^[2] Natl Inst Space Res, Associate Lab Sensors & Mat LABAS, BR-12227010 Sao Jose Dos Campos - Brazil

^[3] Univ Vale Paraiba UNIVAP, Photobiol Appl Hlth Res & Dev Inst IP&D, BR-12244390 Sao Jose Dos Campos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 13, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Photodynamic therapy (PDT) mediated by Fotoenticine(R) (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium Streptococcus mutans. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of mutans streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries. (AU)

• Processo FAPESP: 20/08345-0 - Análise do biofilme microcosmo de cárie dentária após aplicação da terapia fotodinâmica com fotoenticine®
• Beneficiário: Rafael Araújo da Costa Ward
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/06909-4 - Ação da terapia fotodinâmica mediada por fotoenticine® sobre biofilmes microcosmos orais
• Beneficiário: Nathália Maria Ferreira Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica