A sandwich-type electrochemical immunosensor based on Au-rGO composite for CA15-3 tumor marker detection

Autor(es)

Martins, Thiago S. ^[1] ; Bott-Neto, Jose L. ^[2] ; Oliveira, Jr., Osvaldo N. ^[2] ; Machado, Sergio A. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchimica Acta; v. 189, n. 1 JAN 2022.
• Citações Web of Science: 0

Resumo

A sensitive detection of carbohydrate antigen 15-3 (CA15-3) levels may allow for early diagnosis and monitoring the treatment of breast cancer, but this can only be made in routine clinical practice if low-cost immunosensors are available. In this work, we developed a sandwich-type electrochemical immunosensor capable of rapid detection of CA15-3 with an ultra-low limit of detection (LOD) of 0.08 fg mL(-1) within a wide linear concentration range from 0.1 fg mL(-1) to 1 mu g mL(-1). The immunosensor had a matrix of a layer-by-layer film of Au nanoparticles and reduced graphene oxide (Au-rGO) co-electrodeposited on screen-printed carbon electrodes (SPCE). The high sensitivity was achieved by using secondary antibodies (Ab(2)) labeled with horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2) as signal amplifiers, and hydroquinone (HQ) was used as an electron mediator. The immunosensor was selective for CA15-3 in human serum and artificial saliva samples, robust, and stable to permit storage at 4 degrees C for more than 30 days. With its high performance, the immunosensor may be incorporated into future point-of-care (POC) devices to determine CA15-3 in distinct biological fluids, including in blood and saliva samples. (AU)

• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/13514-9 - Sensores eletroquímicos com matriz contendo pontos quânticos ou nanofitas de grafeno para detecção de biomarcadores
• Beneficiário: Jose Luiz Bott Neto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/01777-5 - Confecção de dispositivos eletroquímicos descartáveis compostos por nanoesferas ocas de carbono e nanofios de carbono decorados com nanopartículas de prata para a quantificação de interferentes endócrinos em amostras de água de abastecimento
• Beneficiário: Sergio Antonio Spinola Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular