Antibacterial noncytotoxic chitosan coatings on polytetrafluoroethylene films by plasma grafting for medical device applications

Autor(es)

Vaz, Juliana M. ^[1] ; Taketa, Thiago B. ^[2] ; Hernandez-Montelongo, Jacobo ^[3] ; Fiuza, Larissa M. C. G. ^[1] ; Rodrigues, Cristiano ^[4] ; Beppu, Marisa M. ^[2] ; Vieira, Rodrigo S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Ceara, Dept Chem Engn, Campus Pici Bloco 709, BR-60455760 Fortaleza, Ceara - Brazil

^[2] Univ Estadual Campinas, Sch Chem Engn, Dept Mat Engn & Bioproc, Bldg D, BR-13083852 Campinas, SP - Brazil

^[3] Catholic Univ Temuco, Dept Phys & Math Sci, Bldg CT, Temuco 4813302, La Araucania - Chile

^[4] Fed Univ Hlth Sci Porto Alegre UFCSPA, Dept Basic Hlth Sci, Lab Cell Biol, BR-90050170 Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Coatings Technology and Research; JAN 2022.
• Citações Web of Science: 0

Resumo

Chitosan is an exciting alternative for the development of coating-surfaces due to its large action spectrum against pathogenic microorganisms. However, to produce a stable coating with effective antibacterial action, a compromise between deacetylation degree (DD) and molecular weight (MW) is essential. Four chitosan samples were characterized regarding Mw and DD and correlated with the minimum and bactericide concentrations against E. coli, P. aeruginosa, and S. aureus. CHI80MW (79.7% DD and 7.0 x 10(5) Da) showed the best antibacterial effect and was selected to functionalize polytetrafluoroethylene (PTFE) surfaces by plasma. CHI80MW was grafted onto the PTFE surfaces using two different spacer molecules: poly(ethylene glycol) bis (carboxymethyl) ether (PEG) and poly(ethylene-alt-maleic anhydride) (PA). PTFE-Plasma-PA-CHI80MW exhibited a coating with more attached chitosan and better antibacterial action if compared to PTFE-Plasma-PEG-CHI80MW: after 8 h, PTFE-Plasma-PEG-CHI80MW presented a bacterial reduction of 25-30% for the three bacterial strains, and PTFE-Plasma-PA-CHI80MW reduced them to 77-90%. Moreover, cytotoxicity tests showed that PTFE-Plasma-PA-CHI80MW samples were compatible with human fibroblasts. (AU)

• Processo FAPESP: 13/05135-1 - Recobrimentos antimicrobianos e nanoestruturados contendo quitosana produzidos pela técnica de automontagem (layer-by-layer) para substratos têxteis
• Beneficiário: Thiago Bezerra Taketa
• Modalidade de apoio: Bolsas no Brasil - Doutorado