Brazilwood sawdust composition confirms different feeding habits of larvae and adult males of the ambrosia beetle Megaplatypus mutatus during excavation

Autor(es)

Girardi, Graziele de Souza ^[1] ; da Silva, Claudia Alves ^{[1, 2]} ; Braga, Marcia Regina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim Plantas, Av Miguel Estefano 3687, BR-04301902 Sao Paulo, SP - Brazil

^[2] Secretaria Estadual Educ Sao Paulo, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHYTOPARASITICA; JAN 2022.
• Citações Web of Science: 0

Resumo

Ambrosia beetles are an emerging threat to forestry ecosystems, attacking native and non-native trees. The brazilwood (Paubrasilia echinata (Lam.) Gagnon, H.C.Lima \& G.P.Lewis) is currently considered the most appropriate material for the manufacture of violin bows due to its unique vibrational properties. An experimental plantation of brazilwood has been attacked by the ambrosia beetle Megaplatypus mutatus Chapuis. In this work, we compared the cell wall carbohydrate composition of brazilwood sawdust produced due to the attack of imagoes (adult males) and larvae of this beetle. We aimed to assess changes caused by the insect and its associated microbiota. Cellulose and xylans represented the main fractions of all materials, and pectin accounted for only 10-15% of the walls. The yield of wall fractions and cell wall composition of the sawdust produced by adult beetles, which is composed of long particles, were quite similar to those found in the material obtained from uninfected trunks indicating that the adult male does not feed on the wood at this stage. In contrast, chemical fractionation of the granular sawdust from larvae showed a lower content of extractable pectins, representing only 50% of the total amount detected in the sawdust produced by adult male beetles. Sugar composition analysis of the pectin fractions of the larval sawdust revealed the loss of arabinosyl, galacturonosyl, and galactosyl residues, indicating that the association between beetle/ambrosia fungus evolves towards the degradation of easily accessible pectic polysaccharides of the cell wall. (AU)

• Processo FAPESP: 03/07536-1 - Alteracoes na composicao da parede celular da madeira de pau-brasil ocasionadas pelo ataque da coleobroca platypus sulcatus.
• Beneficiário: Graziele de Souza Girardi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 00/06422-4 - Caesalpinia echinata (Pau-brasil) da semente à madeira, um modelo para estudos de plantas arbóreas tropicais brasileiras
• Beneficiário: Rita de Cassia Leone Figueiredo Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 05/04139-7 - Carboidratos de plantas tropicais como moduladores de processos ecofisiológicos e indicadores de respostas a estresses ambientais
• Beneficiário: Marcia Regina Braga
• Modalidade de apoio: Auxílio à Pesquisa - Temático