Natural latex serum: characterization and biocompatibility assessment using Galleria mellonella as an alternative in vivo model

Autor(es)

Pegorin Brasil, Giovana Sant'Ana ^{[1, 2]} ; de Barros, Patricia Pimentel ^{[3, 4]} ; Miranda, Matheus Carlos Romeiro ^[5] ; de Barros, Natan Roberto ^[6] ; Junqueira, Juliana Campos ^[3] ; Gomez, Alejandro ^[6] ; Herculano, Rondinelli Donizetti ^[2] ; de Mendonca, Ricardo Jose ^[7]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biotechnol & Bioproc Engn, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

^[4] Fed Univ Rio Grande Norte UFRN, Multicampi Sch Med Sci, Caico, RN - Brazil

^[5] Univ Aveiro, Ctr Environm & Marine Studies CESAM, Aveiro - Portugal

^[6] Terasaki Inst Biomed Innovat TIBI, Los Angeles, CA - USA

^[7] Fed Univ Triangulo Mineiro UFTM, Dept Biochem Pharmacol & Physiol, Uberaba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION; DEC 2021.
• Citações Web of Science: 0

Resumo

Natural latex serum (NLS) is one of the natural rubber latex fractions from Hevea brasiliensis tree, which is formed by centrifuged serum and is composed of proteins, acids, nucleotides, salts and carbohydrates. The proteins present in NLS have demonstrated several interesting biological properties, including angiogenic, healing, osteogenic, anti-inflammatory, antimicrobial, in addition to inducing neovascularization, bone formation and osseointegration. Thus, we proposed to characterize NLS by physicochemical techniques and to investigate the biocompatibility by toxicological assays and safety test in Galleria mellonella. Infrared spectrum showed vibrational bands characteristic of amide I, II and III that are linked to the protein content, which was confirmed by the High Performance Liquid Chromatography profile and by the Electrophoresis analysis. This material did not exhibit hemolytic (rate <0.5%) and cytotoxic effects (viability >70%) and was able to enhance the proliferation of fibroblasts (>600%) after 3 days. The pronounced proliferative effect observed in fibroblast cells can be explained by the presence of the fibroblast growth factor (FGF) like protein revealed by the Western blot test. Moreover, NLS did not provoke toxic effects (survival similar to 80%) on the G. mellonella model, indicating that it is a biocompatible and safe material. (AU)

• Processo FAPESP: 14/17526-8 - Desenvolvimento de um novo sistema empregando o látex natural como liberador de extratos vegetais reforçados por nanopartículas: caracterização física, química e biológica
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/17411-8 - Avaliação da liberação controlada de fármacos utilizando membranas de látex natural como carreador
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular