Ultrasensitive magnetogenoassay for detection of microRNA for diagnosis of metastatic lymph nodes in head and neck cancer using disposable electrodes

Autor(es)

Fonseca, Wilson T. ^[1] ; Cincotto, Fernando H. ^[2] ; Lourencao, Bruna C. ^[3] ; de Almeida, V, Sthefane ; Moraes, Fernando C. ^[4] ; Fatibello-Filho, Orlando ^[4] ; de Carvalho, Ana C. ^[5] ; Carvalho, Andre L. ^[5] ; Melendez, Matias E. ^{[5, 6]} ; Faria, Ronaldo C. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Chem Dept, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Inst Chem, Dept Analyt Chem, Rio De Janeiro - Brazil

^[3] Minas Gerais State Univ, BR-38302192 Ituiutaba, MG - Brazil

^[4] de Almeida, Sthefane, V, Univ Fed Sao Carlos, Chem Dept, BR-13565905 Sao Carlos, SP - Brazil

^[5] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil

^[6] Natl Canc Inst, BR-20231050 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SENSORS AND ACTUATORS B-CHEMICAL; v. 352, n. 1 FEB 1 2022.
• Citações Web of Science: 0

Resumo

A fast and simple magnetogenoassay is proposed for diagnosis of metastatic lymph nodes in patients with head and neck squamous cell carcinoma (HNSCC). The developed method involved the construction of a low-cost disposable electrochemical sensor (DES) for the detection of microRNA-203 (miRNA-203) as a biomarker in lymph node samples from patients with HNSCC. A hairpin DNA designed for selectively binding the miRNA-203 was immobilized in magnetic particles (MPs) and used to capture and separate the biomarker from the sample solution. Gold nanoparticles (AuNPs) decorated with single-stranded DNA with a sequence complementary to the hairpin terminal section were prepared and used for biomarker detection. The miRNA-203 magneto-bioconjugate obtained was added to the DES and the biomarker was quantitatively determined by means of the redox properties of the gold present in the AuNPs, using square-wave voltammetry in 0.2 mol L-1 HCl. The proposed method presented a linear range from 1.0 to 500.0 fmol L-1, with a limit of detection of 0.52 fmol L-1. The magnetogenoassay was able to detect the miRNA-203 in 90 min, showing similar results when compared with qRT-PCR for the discrimination of positive and negative metastatic lymph nodes from HNSCC patients. The proposed molecular test requires no amplification steps, offering an effective, simple, and fast alternative for the detection of microRNAs as biomarkers for cancer diagnosis. (AU)

• Processo FAPESP: 16/16565-5 - Desenvolvimento de métodos ultrassensíveis para a detecção de RNA visando o diagnóstico de Câncer de Cabeça e Pescoço
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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• Modalidade de apoio: Auxílio à Pesquisa - Regular